Recombinant人GSK3 beta protein (ab60863)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 序列MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEF MSGRPRTTSF AESCKPVQQP SAFGSMKVSR DKDGSKVTTV VATPGQGPDR PQEVSYTDTK VIGNGSFGVV YQAKLCDSGE LVAIKKVLQD KRFKNRELQI MRKLDHCNIV RLRYFFYSSG EKKDEVYLNL VLDYVPETVY RVARHYSRAK QTLPVIYVKL YMYQLFRSLA YIHSFGICHR DIKPQNLLLD PDTAVLKLCD FGSAKQLVRG EPNVSYICSR YYRAPELIFG ATDYTSSIDV WSAGCVLAEL LLGQPIFPGD SGVDQLVEII KVLGTPTREQ IREMNPNYTE FKFPQIKAHP WTKDSSGTGH FTSGVRVFRP RTPPEAIALC SRLLEYTPTA RLTPLEACAH SFFDELRDPN VKLPNGRDTP ALFNFTTQEL SSNPPLATIL IPPHARIQAA ASTPTNATAA SDANTGDRGQ TNNAASASAS NST
    • 分子量73 kDa
    • 标签GST tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab60863 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific Activity: 187nmol/min/mg

  • 应用

    SDS-PAGE

    Functional Studies

    Western blot

  • 形式Liquid
  • 补充说明

    ab204860 (Glycogen synthase 1 peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Glycogen Synthase Kinase 3 Beta
    • Glycogen synthase kinase-3 beta
    • GSK 3 beta
    • GSK-3 beta
    • GSK3B
    • GSK3B_HUMAN
    • GSK3beta isoform
    • Serine/threonine-protein kinase GSK3B
    see all
  • 功能Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. Phosphorylates CTNNB1/beta-catenin. Phosphorylates SNAI1. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Phosphorylates MACF1 and this phosphorylation inhibits the binding of MACF1 to microtubules which is critical for its role in bulge stem cell migration and skin wound repair.
  • 组织特异性Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney.
  • 序列相似性Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • 翻译后修饰Phosphorylated by AKT1 and ILK1. Activated by phosphorylation at Tyr-216.
  • 细胞定位Cytoplasm. Nucleus. Cell membrane. The phosphorylated form shows localization to cytoplasm and cell membrane. The MEMO1-RHOA-DIAPH1 signaling pathway controls localization of the phosophorylated form to the cell membrane.
  • Information by UniProt

Recombinant human GSK3 beta protein 图像

  • Sample Kinase Activity Plot.
  • ab60863 on SDS-PAGE, MW ~73 kDa.

Recombinant human GSK3 beta protein (ab60863)参考文献

This product has been referenced in:
  • Suryo Rahmanto A  et al. FBW7 suppression leads to SOX9 stabilization and increased malignancy in medulloblastoma. EMBO J 35:2192-2212 (2016). Read more (PubMed: 27625374) »

See 1 Publication for this product

Product Wall

Application Functional Studies
Recombinant human GSK3ß (ab60863) was used to phosphorylate putative GSK3ß substrate (protein X). Evidence of phosphorylation was confirmed by:
(1) in-house generated phospho-specific antibody against the putative substrate (Protein X - T236 data shown)
(2) lambda phosphatase treatment of GSK3ß-treated protein X known phospho-specific protein interactor
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提交于 Dec 08 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"