The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
700 U/mg. One unit is defined as the amount of enzyme that will
phosphorylate 1 nmol of glycogen synthase peptide 2 substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3uM Na-orthovanadate, 0.1 mM ATP, 20 uM substrate, and 0.1 ug recombinant GSK3 alpha.
Expressed in a Baculovirus infected Sf9 cell expression system.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.