Recombinant人Growth Hormone protein (ab51232)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFEEAYIPKE QKYSFLQNPQ TSLCFSESIP TPSNREETQQ KSNLELLRIS LLLIQSWLEP VQFLRSVFAN SLVYGASDSN VYDLLKDLEE GIQTLMGRLE DGSPRTGQIF KQTYSKFDTN SHNDDALLKN YGLLYCFRKD MDKVETFLRI VQCRSVEGSC GF

技术指标

Our Abpromise guarantee covers the use of ab51232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    Recombinant human Growth Hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Endotoxin level < 1.0 EU per 1 microgram of protein (determined by LAL method)
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 88mM Mannitol, 5mM PBS, pH 7.4

常规信息

  • 别名
    • gH
    • GH-N
    • GH1
    • GHN
    • Growth hormone
    • Growth hormone 1
    • Growth hormone, normal
    • Growth hormone, pituitary
    • HG1
    • hGH-N
    • IGHD1B
    • Pituitary growth hormone
    • RNGHGP
    • SOMA_HUMAN
    • Somatotropin
    see all
  • 功能Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
  • 疾病相关Defects in GH1 are a cause of growth hormone deficiency isolated type 1A (IGHD1A) [MIM:262400]; also known as pituitary dwarfism I. IGHD1A is an autosomal recessive deficiency of GH which causes short stature. IGHD1A patients have an absence of GH with severe dwarfism and often develop anti-GH antibodies when given exogenous GH.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 1B (IGHD1B) [MIM:612781]; also known as dwarfism of Sindh. IGHD1B is an autosomal recessive deficiency of GH which causes short stature. IGHD1B patients have low but detectable levels of GH. Dwarfism is less severe than in IGHD1A and patients usually respond well to exogenous GH.
    Defects in GH1 are the cause of Kowarski syndrome (KWKS) [MIM:262650]; also known as pituitary dwarfism VI.
    Defects in GH1 are a cause of growth hormone deficiency isolated type 2 (IGHD2) [MIM:173100]. IGHD2 is an autosomal dominant deficiency of GH which causes short stature. Clinical severity is variable. Patients have a positive response and immunologic tolerance to growth hormone therapy.
  • 序列相似性Belongs to the somatotropin/prolactin family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant Human Growth Hormone protein 图像

  • ab51232 on 14% SDS-PAGE
  • All lanes : Anti-Growth Hormone antibody [GH-1] (ab9821) at 1/500 dilution

    Lane 1 : Recombinant Human Growth Hormone protein (ab51232) at 0.1 µg
    Lane 2 : Recombinant Human Growth Hormone protein (ab51232) at 0.01 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 2 minutes

Recombinant Human Growth Hormone protein (ab51232)参考文献

ab51232 has not yet been referenced specifically in any publications.

Product Wall

ab51232 has not been used for subcutaneous administration in vivo experiments, but pharmacokinetics test has been performed in mouse without any problems. Thus, there shouldn’t be any problems using it for in vivo subcutaneous administration experimen...

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