概述

描述

  • 性质
    Recombinant
  • 来源
    Baculovirus infected Sf9 cells
  • 氨基酸序列
    • Accession
      Q15835
    • 种属
      Human
    • 分子量
      95 kDa
    • 氨基酸
      1 to 563

技术指标

Our Abpromise guarantee covers the use of ab105126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific activity: 4.3 nmol/min/mg
  • 应用

    Western blot

    Functional Studies

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Liquid
  • 补充说明

    It is recommended to store this protein at -80°C in glycerol to preserve ts activity.

     

    ab204870 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EC=2.7.11.14
    • G protein-coupled receptor kinase 1
    • GPRK1
    • Grk1
    • Rhodopsin kinase
    • RHOK
    • RK
    • RK_HUMAN
    see all
  • 功能
    Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
  • 组织特异性
    Retinal-specific. Expressed in rods and cones cells.
  • 疾病相关
    Night blindness, congenital stationary, Oguchi type 2
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    Contains 1 RGS domain.
  • 翻译后修饰
    Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
    Farnesylation is required for full activity.
  • 细胞定位
    Membrane.
  • Information by UniProt

图片

  • Functional Studies - GRK1 protein (ab105126)
    Functional Studies - GRK1 protein (ab105126)
    Kinase Activity Plot. Specific activity is 4.3 nmol/min/mg
  • SDS-PAGE - GRK1 protein (ab105126)
    SDS-PAGE - GRK1 protein (ab105126)
    Recombinant human GRK1 protein (ab105126) + plus proprietary tag

文献

ab105126 has not yet been referenced specifically in any publications.

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