概述
- 产品名称Recombinant人GRK1 protein
参阅全部 GRK1 蛋白酶 - 蛋白长度Full length protein
描述
- 性质Recombinant
- 来源Baculovirus infected Sf9 cells
- 氨基酸序列
- AccessionQ15835
- 种属Human
- 分子量95 kDa
- 氨基酸1 to 563
相关产品
- Related Products
- Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab105126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 生物活性Specific activity: 4.3 nmol/min/mg
- 应用
Western blot
Functional Studies
- 纯度> 95
% SDS-PAGE.
- 形式Liquid
- 补充说明
It is recommended to store this protein at -80°C in glycerol to preserve ts activity.
ab204870 (Cdc25C peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
- 稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: None
Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- EC=2.7.11.14
- G protein-coupled receptor kinase 1
- GPRK1
see all - 功能Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade. This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination.
- 组织特异性Retinal-specific. Expressed in rods and cones cells.
- 疾病相关Night blindness, congenital stationary, Oguchi type 2
- 序列相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 RGS domain. - 翻译后修饰Autophosphorylated, Ser-21 is a minor site of autophosphorylation compared to Ser-491 and Thr-492 (By similarity). Phosphorylation at Ser-21 is regulated by light and activated by cAMP.
Farnesylation is required for full activity. - 细胞定位Membrane.
- Information by UniProt
Recombinant human GRK1 protein 图像
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Functional Studies - GRK1 protein (ab105126)Kinase Activity Plot. Specific activity is 4.3 nmol/min/mg -
SDS-PAGE - GRK1 protein (ab105126)Recombinant human GRK1 protein (ab105126) + plus proprietary tag
Recombinant human GRK1 protein (ab105126)参考文献
ab105126 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"