The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
生物活性ab83993 bound to protein A sepharose beads was able to pull down its ligand, GM-CSF.
补充说明ab83993 bound to protein A sepharose beads was able to pull down its ligand, GM-CSF.
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Shipped at 4°C. Store at +4°C.
Constituents: 10% Trehalose, 1% Human serum albumin
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.
功能Low affinity receptor for granulocyte-macrophage colony-stimulating factor. Transduces a signal that results in the proliferation, differentiation, and functional activation of hematopoietic cells.
疾病相关Defects in CSF2RA are the cause of pulmonary surfactant metabolism dysfunction type 4 (SMDP4) [MIM:300770]. A rare lung disorder due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
序列相似性Belongs to the type I cytokine receptor family. Type 5 subfamily.
结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box 1 motif is required for JAK interaction and/or activation.
Recombinant human GM-CSF Receptor alpha protein (Fc Chimera) 图像
Functional Studies - GM-CSF Receptor alpha - Fc Chimera (ab83993)
Densitometry of protein isoforms visualised by 2-DE.
The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational
The triangle indicates the theoretical MW and pI of the protein.
SDS-PAGE - GM-CSF Receptor alpha - Fc Chimera (ab83993)
1D SDS-PAGE of ab83993 before and after treatment with glycosidases to remove oligosaccharides.
Lane 1: ab83993
Lane 2: ab83993 treated with PNGase F to remove potential N-linked glycans
Lane 3: ab83993 treated with a glycosidase
cocktail to remove potential N- and O-linked glycans. Approximately 5 μg of protein was loaded per lane; Gel was stained using Deep Purple™.
Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
Recombinant human GM-CSF Receptor alpha protein (Fc Chimera) (ab83993)参考文献
has not yet been referenced specifically in any publications.