Recombinant人GM-CSF protein (ab88382)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • 种属Human
    • 序列APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMF D LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPET SC ATQIITFESFKENLKDFLLVIPFDCWEPVQE

技术指标

Our Abpromise guarantee covers the use of ab88382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: The ED50 of ab88382 is typically 0.02 - 0.2 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent TF-1 cell line.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • 功能Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 序列相似性Belongs to the GM-CSF family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human GM-CSF protein 图像

  • 1D SDS-PAGE of ab88382 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab88382
    Lane 2: ab88382 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab88382 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

    Drop in MWt after treatment with PNGase F indicates the presence of N-linked glycans. Subsequent small drop in MWt after treatment with glycosidase cocktail suggests possible presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
  • A sample of ab88382 without carrier protein was reduced and alkylated. 40 µg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot trains (Deep Purple™ stained) indicates the presence of multiple isoforms of GM-CSF.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MWt and pI of the protein.

Recombinant human GM-CSF protein (ab88382)参考文献

ab88382 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The ED50 is an expression of activity at 50% of its maximum response. Specific activity is just another way of conveying the activity of the protein. To calculate a specific activity, divide 1 x 106 by the ED50 in ng/ml...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"