重组人GM-CSF蛋白(ab88382)

概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • 种属
      Human
    • 序列
      APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMF D LQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPET SC ATQIITFESFKENLKDFLLVIPFDCWEPVQE

技术指标

Our Abpromise guarantee covers the use of ab88382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Activity: The ED50 of ab88382 is typically 0.02 - 0.2 ng/ml as measured in a cell proliferation assay using the human growth factor-dependent TF-1 cell line.
  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • Colony stimulating factor
    • Colony Stimulating Factor 2
    • Colony stimulating factor 2 (granulocyte-macrophage)
    • Colony-stimulating factor
    • CSF
    • CSF 2
    • CSF2
    • CSF2_HUMAN
    • GM-CSF
    • GMCSF
    • Granulocyte Macrophage Colony Stimulating Factor
    • Granulocyte-macrophage colony-stimulating factor
    • MGC131935
    • MGC138897
    • MGI1GM
    • Molgramostin
    • Pluripoietin-a
    • Sargramostim
    see all
  • 功能
    Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
  • 序列相似性
    Belongs to the GM-CSF family.
  • 细胞定位
    Secreted.
  • Information by UniProt

图片

  • 1D SDS-PAGE of ab88382 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab88382
    Lane 2: ab88382 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab88382 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

    Drop in MWt after treatment with PNGase F indicates the presence of N-linked glycans. Subsequent small drop in MWt after treatment with glycosidase cocktail suggests possible presence of O-linked glycans. Additional bands in lane 2 and lane 3 are glycosidase enzymes.
  • A sample of ab88382 without carrier protein was reduced and alkylated. 40 µg protein was loaded, focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. Spot trains (Deep Purple™ stained) indicates the presence of multiple isoforms of GM-CSF.
  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MWt and pI of the protein.

文献

ab88382 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for contacting us. The ED50 is an expression of activity at 50% of its maximum response. Specific activity is just another way of conveying the activity of the protein. To calculate a specific activity, divide 1 x 106 by the ED50 in ng/ml...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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