Recombinant人Glutaredoxin 2 protein (ab82665)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 氨基酸
      41 to 164

技术指标

Our Abpromise guarantee covers the use of ab82665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Specific activity : 45 U/mg One unit will cause the oxidation of 1µmole of NADPH per min.
  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM HEPES, pH 7

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • bA101E13.1
    • bA101E13.1 (GRX2 glutaredoxin (thioltransferase) 2)
    • CGI133
    • Glrx2
    • GLRX2_HUMAN
    • Glutaredoxin-2
    • Glutaredoxin-2, mitochondrial
    • GRX2
    • mitochondrial
    see all
  • 功能
    Glutathione-dependent oxidoreductase that facilitates the maintenance of mitochondrial redox homeostasis upon induction of apoptosis by oxidative stress. Involved in response to hydrogen peroxide and regulation of apoptosis caused by oxidative stress. Acts as a very efficient catalyst of monothiol reactions because of its high affinity for protein glutathione-mixed disulfides. Can receive electrons not only from glutathione (GSH), but also from thioredoxin reductase supporting both monothiol and dithiol reactions. Efficiently catalyzes both glutathionylation and deglutathionylation of mitochondrial complex I, which in turn regulates the superoxide production by the complex. Overexpression decreases the susceptibility to apoptosis and prevents loss of cardiolipin and cytochrome c release.
  • 组织特异性
    Widely expressed. Expressed in brain, heart, skeletal muscle, colon, thymus, spleen, kidney, liver, small intestine, placenta and lung. Not expressed in peripheral blood leukocytes.
  • 序列相似性
    Belongs to the glutaredoxin family.
    Contains 1 glutaredoxin domain.
  • 细胞定位
    Mitochondrion and Nucleus.
  • Information by UniProt

图片

  • ab82665 at 1µg on SDS-PAGE.

文献

ab82665 has not yet been referenced specifically in any publications.

客户评价及客户问答

Here is how the lab performs the activity assay:


100 mM Tris-Cl(pH8.0), 2 mM EDTA, 0.1 mg/ml BSA, 1 mM GSH, 0.2 mM NADPH, 6 ug/ml yeast GR, 0.7 mM HED and X ug Grx2 protein.


Here are some other publications describing measur...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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