Recombinant人Glucose 6 phosphate isomerase protein (ab87625)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ

技术指标

Our Abpromise guarantee covers the use of ab87625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度> 95 % SDS-PAGE.
    ab87625 is purified using conventional chromatography techniques. Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • AMF
    • Aurocrine motility factor
    • Autocrine motility factor
    • DKFZp686C13233
    • EC 5.3.1.9
    • G6PI_HUMAN
    • Glucose phosphate isomerase
    • Glucose-6-phosphate isomerase
    • GNPI
    • GPI
    • Gpi1
    • Hexose monophosphate isomerase
    • Hexosephosphate isomerase
    • Neuroleukin
    • NLK
    • Oxoisomerase
    • PGI
    • PHI
    • Phosphoglucose isomerase
    • Phosphohexomutase
    • Phosphohexose isomerase
    • Phosphosaccharomutase
    • SA 36
    • SA-36
    • SA36
    • Sperm antigen 36
    see all
  • 功能Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • 通路Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.
  • 疾病相关Defects in GPI are the cause of hemolytic anemia non-spherocytic due to glucose phosphate isomerase deficiency (HA-GPID) [MIM:613470]. It is a form of anemia in which there is no abnormal hemoglobin or spherocytosis. It is caused by glucose phosphate isomerase deficiency. Severe GPI deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
  • 序列相似性Belongs to the GPI family.
  • 翻译后修饰Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.
    ISGylated.
  • 细胞定位Cytoplasm. Secreted.
  • Information by UniProt

Recombinant Human Glucose 6 phosphate isomerase protein 图像

  • 15% SDS-PAGE showing ab87625 at approximately 65kDa (3µg).
  • All lanes : Anti-Glucose 6 phosphate isomerase antibody (ab86950) at 1 µg/ml

    Lane 1 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.1 µg
    Lane 2 : Recombinant Human Glucose 6 phosphate isomerase protein (ab87625) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant Human Glucose 6 phosphate isomerase protein (ab87625)参考文献

This product has been referenced in:
  • Zong M  et al. Glucose-6-phosphate isomerase promotes the proliferation and inhibits the apoptosis in fibroblast-like synoviocytes in rheumatoid arthritis. Arthritis Res Ther 17:100 (2015). Read more (PubMed: 25889507) »

See 1 Publication for this product

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