Recombinant人GDF8 / Myostatin protein (ab73251)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionO14793
    • 种属Human
    • 序列The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp.
    • 额外的序列信息The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Phe-Gly-Leu-Asp so starting at aa 267.

相关产品

技术指标

Our Abpromise guarantee covers the use of ab73251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    The ED50 as determined by the inhibition of the proliferation of MPC-11 cells is < 20ng/ml, corresponding to a Specific Activity of 50,000units/mg.

  • 应用

    Western blot

    Functional Studies

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    This product is purified by proprietary chromatographic techniques. Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
  • 形式Lyophilised
  • 补充说明For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None.
    Additives: None.

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶Reconstitute in sterile 20mM HCl at 0.1 mg/ml, which can then be further diluted to other aqueous solutions.

常规信息

  • 别名
    • GDF 8
    • GDF-8
    • GDF8
    • GDF8_HUMAN
    • Growth differentiation factor 8
    • Growth/differentiation factor 8
    • MSLHP
    • MSTN
    • Myostatin
    • OTTHUMP00000163498
    see all
  • 功能Acts specifically as a negative regulator of skeletal muscle growth.
  • 序列相似性Belongs to the TGF-beta family.
  • 细胞定位Secreted.
  • Information by UniProt

Recombinant human GDF8 / Myostatin protein (ab73251)参考文献

ab73251 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us.

We would suggest to reconstitute the sample in 20 mM HCL prior to diluting it. For our assay, the myostatin was not replenished everyday. Also, we are not sure of the stability in culture, but our assays are run f...

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Thank you for contacting us.

These two proteins are the same, except for the reconstitution instructions (water versus 20 mM Hcl). Both should work equally well in inhiibiting MPC-11 cell growth.

I hope this information is helpful to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"