The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
% SDS-PAGE. ab117474 is purified by proprietary chromatographic techniques.
Lyophilized ab117474, although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ab117474 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
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Shipped at 4°C. Please see notes section.
It is recommended to reconstitute in sterile 100mM Acetate buffer pH4 at a concentration of 0.5mg/ml. For the dilution into higher pH values, it is recommended to dilute the protein to a concentration of 10µg/ml. Please note that in higher concentrations the solubility of ab117474 is limited. The protein is not sterile! Please sterile filter before using it in the cell culture.