重组人GDF15蛋白(ab50077)
Key features and details
- Expression system: CHO cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人GDF15蛋白
参阅全部 GDF15 蛋白酶 -
生物活性
Determined by its ability to inhibit alkaline phosphatase activity in differentiating MC3T3/E1 osetoblast cells. The expected ED50 for this effect is 75-200 ng/ml.
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纯度
> 95 % SDS-PAGE.
ab50077 purity was determined also by HPLC -
内毒素水平
< 1.000 Eu/µg -
表达系统
CHO cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
ARNGDHCPLGPGRCCRLHTVRASLEDLGWADWVLSPREVQVTMCIGACPS QFRAANMHAQIKTSLHRLKPDTVPAPCCVPASYNPMVLIQKTDTGVSLQT YDDLLAKDCHCI -
预测分子量
25 kDa -
实际分子量
25 kDa -
氨基酸
197 to 308 -
额外的序列信息
This product refers to the processed mature form of this protein from aa 195 to 308. Therefore it does not contain the signal peptide or propeptide.
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技术指标
Our Abpromise guarantee covers the use of ab50077 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
Molecular Weight: 24.6 kDa
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute to 1mg/ml with distilled water.
常规信息
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别名
- GDF 15
- GDF-15
- Gdf15
see all -
组织特异性
Highly expressed in placenta, with lower levels in prostate and colon and some expression in kidney. -
序列相似性
Belongs to the TGF-beta family. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab50077 被引用在 3 文献中.
- Radwanska A et al. Increased expression and accumulation of GDF15 in IPF extracellular matrix contribute to fibrosis. JCI Insight 7:N/A (2022). PubMed: 35993367
- Shao M et al. Artemisinin analog SM934 alleviates epithelial barrier dysfunction via inhibiting apoptosis and caspase-1-mediated pyroptosis in experimental colitis. Front Pharmacol 13:849014 (2022). PubMed: 36120344
- Hao T et al. LINC-PINT suppresses tumour cell proliferation, migration and invasion through targeting miR-374a-5p in ovarian cancer. Cell Biochem Funct 38:1089-1099 (2020). PubMed: 32638404