Recombinant人GCSF Receptor protein (Fc Chimera) (ab83994)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionQ99062
    • 种属Human
    • 序列Theoretical Sequence: ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPG GRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAG YPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQ TQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQ LCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQ KCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRW PLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVP LEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVAL VAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWP QGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIV TPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPP ELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHL MAASQAGATNSTVLTLMTLTPRSSNTKVDKKVEPKSCDKTHTCPPCPA PELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYV DGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKA LPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPS DIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVF SCSVMHEALHNHYTQKSLSLSPGK
    • 氨基酸25 to 621
    • 额外的序列信息Encodes the signal peptide and extracellular domain of human G-CSF R (aa 1-621) was fused to the Fc region of human IgG1 (aa 90-330). The chimeric protein was expressed in modified human 293 cells.

技术指标

Our Abpromise guarantee covers the use of ab83994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The ED50 of ab83994 is typically 2-3 ng/ml as measured by its ability to neutralize GCSF mediated proliferation of the murine myeloblastic m-NFS-60 cell line.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • CD 114
    • CD114
    • CD114 antigen
    • Colony stimulating factor 3 receptor
    • Colony stimulating factor 3 receptor (granulocyte)
    • CSF 3R
    • CSF3R
    • CSF3R_HUMAN
    • Csfgr
    • G CSF R
    • G-CSF receptor
    • G-CSF-R
    • GCSFR
    • Granulocyte colony stimulating factor receptor
    • Granulocyte colony-stimulating factor receptor
    • OTTHUMP00000009703
    • OTTHUMP00000009704
    • OTTHUMP00000009705
    see all
  • 功能Receptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
  • 组织特异性One or several isoforms have been found in myelogenous leukemia cell line KG-1, leukemia U-937 cell line, in bone marrow cells, placenta, and peripheral blood granulocytes. Isoform GCSFR-2 is found only in leukemia U-937 cells. Isoform GCSFR-3 is highly expressed in placenta.
  • 疾病相关Hereditary neutrophilia
  • 序列相似性Belongs to the type I cytokine receptor family. Type 2 subfamily.
    Contains 5 fibronectin type-III domains.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
  • 结构域The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
    The box 1 motif is required for JAK interaction and/or activation.
  • 细胞定位Secreted and Cell membrane.
  • Information by UniProt

Recombinant human GCSF Receptor protein (Fc Chimera) 图像

  • 1D SDS-PAGE of ab83994 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab83994
    Lane 2: ab83994 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab83994 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    Approximately 5 µg of protein was loaded per lane; Gel was stained using Coomassie.

    Drop in MW after treatment with PNGase F indicates presence of N-linked glycans. A tightening of the band after treatment with the glycosidase cocktail indicates that O-linked glycans may be present. Additional bands in lane 2 and lane 3 are glycosidase enzymes.

Recombinant human GCSF Receptor protein (Fc Chimera) (ab83994)参考文献

ab83994 has not yet been referenced specifically in any publications.

Product Wall


Each separate size is filled separately so that the target amount of protein of the primary product is maintained.
When each is brought to 0.5ml, each will have only 1% HSA and 10% sugar as a stabilizer. The 25ug size will be 25ug/500ul and t...

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"We have our specification that was used to pass the current batch of material. The material would not have been released if it had not worked at the time of release and I've never had any complaints or reason to suspect otherwise.
Our sp...

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The source of the GCSF Receptor protein ab83994 informed me that :
With regards to any of our proteins actually binding HSA, no we do not have any data, and I suppose one could get some minor binding just due to protein-protein interactions s...

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Thank you for your inquiry.

I can confirm that 10% Trehalose and 1% Human serum albumin are contained in the lyophilized product.

Please follow the instructions on the datasheet for reconstitution.

I hope this information ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"