概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 氨基酸
      1 to 335

技术指标

Our Abpromise guarantee covers the use of ab82633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性

    Specific activity : up to 30U/mg One unit of activity of GAPDH is defined as the amount of enzyme required to convert 1µmole of NAD to NADH per min under standard assay conditions.

  • 应用

    SDS-PAGE

    Western blot

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM HEPES, pH 7.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    Reconstitute with HEPES or Tris buffer or with 260 ul of distilled water. Add 10% glycerol for longer term storage.

常规信息

  • 别名
    • 38 kDa BFA-dependent ADP-ribosylation substrate
    • aging associated gene 9 protein
    • Aging-associated gene 9 protein
    • BARS-38
    • cb609
    • EC 1.2.1.12
    • Epididymis secretory sperm binding protein Li 162eP
    • G3P_HUMAN
    • G3PD
    • G3PDH
    • GAPD
    • GAPDH
    • Glyceraldehyde 3 phosphate dehydrogenase
    • Glyceraldehyde-3-phosphate dehydrogenase
    • HEL-S-162eP
    • KNC-NDS6
    • MGC102544
    • MGC102546
    • MGC103190
    • MGC103191
    • MGC105239
    • MGC127711
    • MGC88685
    • OCAS, p38 component
    • OCT1 coactivator in S phase, 38-KD component
    • peptidyl cysteine S nitrosylase GAPDH
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • wu:fb33a10
    see all
  • 功能
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性
    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位
    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt

图片

  • ab82633 (1µg) on SDS-PAGE.

文献

This product has been referenced in:
  • Deng J  et al. Long non-coding RNA OIP5-AS1 functions as an oncogene in lung adenocarcinoma through targeting miR-448/Bcl-2. Biomed Pharmacother 98:102-110 (2018). WB ; Human . Read more (PubMed: 29247949) »

See 1 Publication for this product

客户评价及客户问答



We would recommend reconstituting the GAPDH protein (Active), ab82633, with 260 uL of distilled sterilised water. This will produce a buffer of 20mM HEPES, pH 7.0. 10% glycerol can be added if longer term storage is required.
260µl ...

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I have checked with the lab and they have confirmed that this protein is not tagged. It was purified by ion exchange.

We would recommend reconstituting the GAPDH protein (Active), ab82633, with 260 uL of distilled sterilised water. This will produce a buffer of 20mM HEPES, pH 7.4. 10% glycerol can be added if longer term storage is required.

The GAPDH protein ab82633 and ab77109 are both the full length GAPDH expressed in E. coli, however only ab82633 has been tested for its enzymatic activity. The specific activity was found to be 35.7 U/mg. One unit of activity of GAPDH is defined as the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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