Recombinant人GAPDH protein (ab77109)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP04406
    • 种属Human
    • 序列MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
    • 氨基酸1 to 335

技术指标

Our Abpromise guarantee covers the use of ab77109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    ab77109 is purified by using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris, 1mM DTT, 1mM EDTA, pH 8.0

常规信息

  • 别名
    • 38 kDa BFA-dependent ADP-ribosylation substrate
    • aging associated gene 9 protein
    • Aging-associated gene 9 protein
    • BARS-38
    • cb609
    • EC 1.2.1.12
    • Epididymis secretory sperm binding protein Li 162eP
    • G3P_HUMAN
    • G3PD
    • G3PDH
    • GAPD
    • GAPDH
    • Glyceraldehyde 3 phosphate dehydrogenase
    • Glyceraldehyde-3-phosphate dehydrogenase
    • HEL-S-162eP
    • KNC-NDS6
    • MGC102544
    • MGC102546
    • MGC103190
    • MGC103191
    • MGC105239
    • MGC127711
    • MGC88685
    • OCAS, p38 component
    • OCT1 coactivator in S phase, 38-KD component
    • peptidyl cysteine S nitrosylase GAPDH
    • Peptidyl-cysteine S-nitrosylase GAPDH
    • wu:fb33a10
    see all
  • 功能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • 通路Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 序列相似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻译后修饰S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt

Recombinant Human GAPDH protein 图像

  • SDS-PAGE showing 3ug of ab77109 migrating at approximately 36kDa.

Recombinant Human GAPDH protein (ab77109)参考文献

This product has been referenced in:
  • Backlund M  et al. Endoplasmic reticulum stress increases AT1R mRNA expression via TIA-1-dependent mechanism. Nucleic Acids Res 44:3095-104 (2016). Human . Read more (PubMed: 26681690) »

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
It is very good loading control for Human samples. I tried it on Human Brain tissue samples and it showed very nice specific bands at around 38KDa
Username

Abcam user community

Verified customer

提交于 Jun 05 2015

Thank you for contacting us.

As we discussed over the phone, could you please send me an image of your microchip/SDS-PAGE data, so that the lab can have a look as well for a posible explanation of the 10 kD a band that you are seeing?
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Vielen Dank für Ihren Anruf.

Es tut mir leid, dass Sie Probleme mit unserem GAPDH-Antikörper bzw. -Protein hatten. Wie am Telefon besprochen, habe ich eine kostenlose Ersatzlieferung mit ab8245 für Sie in Auftrag gegeben. Sie hat die Referen...

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Thank you again for contacting us and sorry for the delay in getting back to you.
The Glutaredoxin 1 protein (Active), ab63231, is the Glutaredoxin 1 protein from Saccharomyces cerevisiae (SwissProt reference P25373).
The activity of the prot...

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Thank you for contacting us and your interest in our products.

The GAPDH protein ab82633 and ab77109 are both the full length GAPDH expressed in E. coli, however only ab82633 has been tested for its enzymatic activity. The specific activity ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"