Recombinant人gamma Synuclein protein (ab48712)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD
    • 氨基酸1 to 127

技术指标

Our Abpromise guarantee covers the use of ab48712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度> 95 % SDS-PAGE.
    The protein coding region of ß-Synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. ß-Synuclein was overexpressed in E. coli and purified to apparent homogeneity by taking advantage of the thermosolubility of the protein and by using conventional column chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 0.1M Sodium chloride, 20mM Tris HCl, pH 7.5

常规信息

  • 别名
    • BCSG1
    • Breast cancer specific gene 1 protein
    • Breast cancer specific protein 1
    • Breast cancer-specific gene 1 protein
    • Gamma synuclein
    • Gamma-synuclein
    • Persyn
    • PRSN
    • SNCG
    • SR
    • Synoretin
    • Synuclein gamma
    • synuclein, gamma (breast cancer-specific protein 1)
    • SYUG_HUMAN
    see all
  • 功能Plays a role in neurofilament network integrity. May be involved in modulating axonal architecture during development and in the adult. In vitro, increases the susceptibility of neurofilament-H to calcium-dependent proteases (By similarity). May also function in modulating the keratin network in skin. Activates the MAPK and Elk-1 signal transduction pathway.
  • 组织特异性Highly expressed in brain, particularly in the substantia nigra. Also expressed in the corpus callosum, heart, skeletal muscle, ovary, testis, colon and spleen. Weak expression in pancreas, kidney and lung.
  • 序列相似性Belongs to the synuclein family.
  • 翻译后修饰Phosphorylated. Phosphorylation by GRK5 appears to occur on residues distinct from the residue phosphorylated by other kinases.
  • 细胞定位Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle. Associated with centrosomes in several interphase cells. In mitotic cells, localized to the poles of the spindle.
  • Information by UniProt

Recombinant Human gamma Synuclein protein 图像

  • 15% SDS-PAGE gel loaded with recombinant gamma Synuclein protein.
  • Anti-gamma Synuclein antibody (ab46201) at 0.5 µg/ml + Recombinant Human gamma Synuclein protein (ab48712) at 0.05 µg

    Secondary
    Goat polyclonal to rabbit IgG - H&L - Pre adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size : 15 kDa (why is the actual band size different from the predicted?)

Recombinant Human gamma Synuclein protein (ab48712)参考文献

This product has been referenced in:
  • Surgucheva I  et al. ?-Synuclein: seeding of a-synuclein aggregation and transmission between cells. Biochemistry 51:4743-54 (2012). Read more (PubMed: 22620680) »

See 1 Publication for this product

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