MTLEEFSAGE QKTERMDKVG DALEEVLSKA LSQRTITVGV YEAAKLLNVD PDNVVLCLLA ADEDDDRDVA LQIHFTLIQA FCCENDINIL
RVSNPGRLAE LLLLETDAGP AASEGAEQPP DLHCVLVTNP HSSQWKDPAL SQLICFCRES RYMDQWVPVI NLPERLEHHH HHH
19 kDa including tags
1 to 165
His tag C-Terminus
GADD45A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids (1-165 a.a.) and having a molecular mass of 19.4kDa.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab117181 is purified by proprietary chromatographic techniques. Purity is greater than 85.0% as determined by SDS-PAGE.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Concentration information loading... 制备和贮存
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituents: 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride
Binds to proliferating cell nuclear antigen. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase.
Belongs to the GADD45 family.
Information by UniProt
Recombinant Human GADD45A protein (ab117181)参考文献
has not yet been referenced specifically in any publications.
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