Recombinant人GABARAPL1 protein (ab101641)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9H0R8
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMKFQYKEDHPFEYRKKEGEKIRKKYPDRVP VIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFV NNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK
    • 分子量16 kDa including tags
    • 氨基酸1 to 117
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 85 % SDS-PAGE.
    ab101641 was purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • APG8 like
    • APG8L
    • ATG8
    • ATG8B
    • ATG8L
    • Early estrogen regulated protein
    • Early estrogen-regulated protein
    • GABA
    • GABA(A) receptor associated protein like 1
    • GABA(A) receptor-associated protein-like 1
    • GABARAPL1
    • GABARAPL1 a
    • Gamma aminobutyric acid receptor associated protein like 1
    • Gamma-aminobutyric acid receptor-associated protein-like 1
    • GBRL1_HUMAN
    • GEC 1
    • GEC-1
    • GEC1
    • Glandular epithelial cell protein 1
    see all
  • 功能Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation.
  • 组织特异性Ubiquitous. Expressed at very high levels in the brain, heart, peripheral blood leukocytes, liver, kidney, placenta and skeletal muscle. Expressed at very low levels in thymus and small intestine. In the brain, expression is particularly intense in motoneurons in the embryo and in neurons involved in somatomotor and neuroendocrine functions in the adult, particularly in the substantia nigra pars compacta.
  • 序列相似性Belongs to the ATG8 family.
  • 翻译后修饰The precursor molecule is cleaved by ATG4B to form the cytosolic form, GABARAPL1-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, GABARAPL1-II (By similarity). ATG4B also mediates the delipidation required for GABARAPL1 recycling when autophagosomes fuse with lysosomes.
    The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine.
  • 细胞定位Cytoplasm > cytoskeleton. Cytoplasmic vesicle membrane. Endoplasmic reticulum. Golgi apparatus. Cytoplasmic vesicle > autophagosome.
  • Information by UniProt

Recombinant Human GABARAPL1 protein 图像

  • ab101641 at 3 µg analysed by 15% SDS PAGE.

Recombinant Human GABARAPL1 protein (ab101641)参考文献

ab101641 has not yet been referenced specifically in any publications.

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