Recombinant人G3BP protein (ab103304)

概述

  • 产品名称Recombinant人G3BP protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ13283
    • 种属Human
    • 序列MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGLDSNGK PADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSN NNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESE EEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEP EPEQEPVSEIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQEDLRTFS WASVTSKNLPPSGAVPVTGIPPHVVKVPASQPRPESKPESQIPPQRPQRD QRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSHQLFIGNLPHE VDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRPI MFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGM VQKPGFGVGRGLAPRQVEHHHHHH
    • 分子量53 kDa including tags
    • 氨基酸1 to 466
    • 标签His tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab103304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 85 % SDS-PAGE.
    ab103304 was purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8.0

常规信息

  • 别名
    • ATP dependent DNA helicase VIII
    • ATP-dependent DNA helicase VIII
    • G3BP
    • G3BP-1
    • G3bp1
    • G3BP1_HUMAN
    • GAP binding protein
    • GAP SH3 domain binding protein 1
    • GAP SH3 domain-binding protein 1
    • GTPase activating protein (SH3 domain) binding protein 1
    • hDH VIII
    • Human DNA helicase VIII
    • MGC111040
    • Ras GTPase activating protein binding protein 1
    • Ras GTPase activating protein SH3 domain binding protein
    • Ras GTPase-activating protein-binding protein 1
    • RasGAP associated endoribonuclease G3BP
    see all
  • 功能May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
  • 组织特异性Ubiquitous.
  • 序列相似性Contains 1 NTF2 domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • 结构域The NTF2 domain mediates multimerization.
  • 翻译后修饰Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
    Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
  • 细胞定位Cytoplasm. Cytoplasm > cytosol. Cell membrane. Nucleus. Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells (By similarity). Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
  • Information by UniProt

Recombinant Human G3BP protein 图像

  • 15% SDS-PAGE analysis of 3µg ab103304.

Recombinant Human G3BP protein (ab103304)参考文献

ab103304 has not yet been referenced specifically in any publications.

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