Recombinant人FTO protein (ab109039)

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概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
      Q9C0B1
    • 种属
      Human
    • 分子量
      65 kDa including tags
    • 氨基酸
      2 to 505
    • 标签
      His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab109039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 内毒素水平
    < 1.000 Eu/µg
  • 纯度
    > 90 % SDS-PAGE.
    ab109039 is 0.2µm filtered.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 55mM Tris HCl, 150mM Sodium chloride, pH 8.2

常规信息

  • 别名
    • AlkB homolog 9
    • ALKBH9
    • Alpha-ketoglutarate-dependent dioxygenase FTO
    • AW743446
    • Fat mass and obesity-associated protein
    • FATSO, MOUSE, HOMOLOG OF
    • Fto
    • FTO_HUMAN
    • GDFD
    • KIAA1752
    • mKIAA1752
    • Protein fatso
    see all
  • 功能
    Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.
  • 组织特异性
    Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.
  • 疾病相关
    Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed.
  • 序列相似性
    Belongs to the fto family.
  • 结构域
    The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
  • 细胞定位
    Nucleus.
  • Information by UniProt

Recombinant Human FTO protein (ab109039)参考文献

ab109039 has not yet been referenced specifically in any publications.

发表研究结果有使用 ab109039？请让我们知道，以便我们可以引用本数据表中的参考文章。

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1. Has there been any activity testing done with this protein?
2. Is it possible to cleave off the His tag? For example, is there a protease cleavage site designed for removal of the tag?

At this time we have not determined it's enzymatic activity. Also, cleavage of the His-tag is not possible.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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