重组人FTO蛋白(ab109039)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人FTO蛋白
参阅全部 FTO 蛋白酶 -
纯度
> 90 % SDS-PAGE.
ab109039 is 0.2µm filtered. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
KRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLIL REASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGN PGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETI QALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNV TLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRSAVAVYSYSCEG PEEESEDDSHLEGRDPDIWHVGFKISWDIETPGLAIPLHQGDCYFMLDDL NATHQHCVLAGSQPRFSSTHRVAECSTGTLDYILQRCQLALQNVCDDVDN DDVSLKSFEPAVLKQGEEIHNEVEFEWLRQFWFQGNRYRKCTDWWCQPMA QLEALWKKMEGVTNAVLHEVKREGLPVEQRNEILTAILASLTARQNLRRE WHARCQSRIARTLPADQKPECRPYWEKDDASMPLPFDLTDIVSELRGQLL EAKP -
预测分子量
65 kDa including tags -
氨基酸
2 to 505 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab109039 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 8.20
Constituents: 0.869% Tris HCl, 0.87% Sodium chloride
常规信息
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别名
- AlkB homolog 9
- ALKBH9
- Alpha-ketoglutarate-dependent dioxygenase FTO
see all -
功能
Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation. -
组织特异性
Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary. -
疾病相关
Defects in FTO are the cause of growth retardation developmental delay coarse facies and early death (GRDDCFED) [MIM:612938]. The disease consists of a severe children multiple congenital anomaly syndrome with death by the age of 3 years. All affected individuals had postnatal growth retardation, microcephaly, severe psychomotor delay, functional brain deficits, and characteristic facial dysmorphism. In some patients, structural brain malformations, cardiac defects, genital anomalies, and cleft palate were also observed. -
序列相似性
Belongs to the fto family. -
结构域
The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases. -
细胞定位
Nucleus. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (2)
ab109039 被引用在 2 文献中.
- Sun K et al. Saikosaponin D exhibits anti-leukemic activity by targeting FTO/m6A signaling. Theranostics 11:5831-5846 (2021). PubMed: 33897884
- Su R et al. R-2HG Exhibits Anti-tumor Activity by Targeting FTO/m6A/MYC/CEBPA Signaling. Cell 172:90-105.e23 (2018). PubMed: 29249359