Recombinant人FOXA1 protein (ab98301)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      49 kDa
    • 氨基酸
      1 to 472

技术指标

Our Abpromise guarantee covers the use of ab98301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 纯度
    >= 80 % SDS-PAGE.
    ab98301 was expressed in E. coli and refolded to soluble form.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50mM HEPES, 5mM Calcium chloride, 3mM DTT, 500 mM L-arginine, 300 mM guanidine-HCl, 5 mM cystamine, 5 mM cysteamine, pH 7.5

常规信息

  • 别名
    • forkhead box A1
    • Forkhead box protein A1
    • FOX A1
    • FOXA1
    • FOXA1_HUMAN
    • hepatocyte nuclear factor 3 alpha
    • Hepatocyte nuclear factor 3-alpha
    • HNF 3A
    • HNF-3-alpha
    • HNF-3A
    • HNF3A
    • MGC33105
    • TCF 3A
    • TCF-3A
    • TCF3A
    • Transcription factor 3A
    see all
  • 功能
    Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally discribed as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
  • 组织特异性
    Highly expressed in prostate and ESR1-positive breast tumors. Overexpressed in esophageal and lung adenocarcinomas.
  • 序列相似性
    Contains 1 fork-head DNA-binding domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt

图片

  • SDS-PAGE anaylsis of 5µg ab98301 stained with Coomassie dye.

文献

ab98301 has not yet been referenced specifically in any publications.

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