Recombinant人Flt3 / CD135 protein (Fc Chimera Active) (ab83998)

概述

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionP36888
    • 种属Human
    • 序列Theoretical Sequence: NQDLPVIKCVLINHKNNDSSVGKSSSYPMVSESPEDLGC ALRPQSSGTVYEAAAVEVDVSASITLQVL VDAPGNISCLWVFKHSSLN CQPHFDLQNRGVVSMVILKMTETQAGEYLLFIQSEATNYTILFTVSIRNT L LYTLRRPYFRKMENQDALVCISESVPEPIVEWVLCDSQGESCKEESP AVVKKEEKVLHELFGTDIRCC ARNELGRECTRLFTIDLNQTPQTTLPQ LFLKVGEPLWIRCKAVHVNHGFGLTWELENKALEEGNYFEM STYSTNR TMIRILFAFVSSVARNDTGYYTCSSSKHPSQSALVTIVEKGFINATNSSE DYEIDQYEEFCFS VRFKAYPQIRCTWTFSRKSFPCEQKGLDNGYSISK FCNHKHQPGEYIFHAENDDAQFTKMFTLNIRRK PQVLAEASASQASCF SDGYPLPSWTWKKCSDKSPNCTEEITEGVWNRKANRKVFGQWVSSSTLNM SEAIKGFLVKCCAYNSLGTSCETILLNSPGPFPFIQDRIPKVDKKVEP KSCDKTHTCPPCPAPELLGGPS VFLFPPKPKDTLMISRTPEVTCVVVD VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVL TVLHQDW LNGKEYKCRVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQV SLTCLVKGFYP SDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLT VDKSRWQQGNVFSCSVMHEALHNHYTQKS LSLSPGK
    • 额外的序列信息Fused with the Fc region of Human IgG1 at the C-terminus.

技术指标

Our Abpromise guarantee covers the use of ab83998 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: The ED50 of ab83998 is typically 1.0 -2.0 ng/ml as measured by its ability to neutralize Flt3 mediated proliferation of the OCI/AML5 human leukemia cell line.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 95 % SDS-PAGE.

  • 形式Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • CD 135
    • CD135
    • CD135 antigen
    • Fetal liver kinase 2
    • FL cytokine receptor
    • Flk 2
    • Flk2
    • Flt 3
    • FLT-3
    • Flt3
    • FLT3_HUMAN
    • FMS like tyrosine kinase 3
    • Fms related tyrosine kinase 3
    • Fms-like tyrosine kinase 3
    • Growth factor receptor tyrosine kinase type III
    • Ly-72
    • OTTHUMP0000004234
    • Receptor type tyrosine protein kinase FLT3
    • Stem cell tyrosine kinase 1
    • Stk 1
    • STK-1
    • Stk1
    • Tyrosine protein kinase receptor FLT3
    • Tyrosine-protein kinase receptor FLT3
    see all
  • 功能Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
  • 组织特异性Bone marrow cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human Flt3 / CD135 protein (Fc Chimera Active) 图像

  • Densitometry of protein isoforms visualised by 2-DE.
    The densitometry scan demonstrates the purified human cell expressed protein exists in multiple isoforms, which differ according to their level of post-translational modification.
    The triangle indicates the theoretical MW and pI of the protein.
  • 1D SDS-PAGE of ab83998 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab83998
    Lane 2: ab83998 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab83998 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.
    10 µg protein loaded per lane; Deep Purple™ stained.
  • A sample of ab83998 without carrier protein was reduced and alkylated and focused on a 3-10 IPG strip then run on a 4-20% Tris-HCl 2D gel. 40 µg protein loaded. Spot train indicates presence of multiple isoforms.

Recombinant human Flt3 / CD135 protein (Fc Chimera Active) (ab83998)参考文献

ab83998 has not yet been referenced specifically in any publications.

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