Recombinant人FKBP52 protein (ab84189)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MTAEEMKATE SGAQSAPLPM EGVDISPKQD EGVLKVIKRE GTGTEMPMIG DRVFVHYTGW LLDGTKFDSS LDRKDKFSFD LGKGEVIKAW DIAIATMKVG EVCHITCKPE YAYGSAGSPP KIPPNATLVF EVELFEFKGE DLTEEEDGGI IRRIQTRGEG YAKPNEGAIV EVALEGYYKD KLFDQRELRF EIGEGENLDL PYGLERAIQR MEKGEHSIVY LKPSYAFGSV GKEKFQIPPN AELKYELHLK SFEKAKESWE MNSEEKLEQS TIVKERGTVY FKEGKYKQAL LQYKKIVSWL EYESSFSNEE AQKAQALRLA SHLNLAMCHL KLQAFSAAIE SCNKALELDS NNEKGLFRRG EAHLAVNDFE LARADFQKVL QLYPNNKAAK TQLAVCQQRI RRQLAREKKL YANMFERLAE EENKAKAEAS SGDHPTDTEM KEEQKSNTAG SQSQVETEA

技术指标

Our Abpromise guarantee covers the use of ab84189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH 8.0 using chymotrypsin.

    Activity Assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP4 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • 应用

    SDS-PAGE

    Functional Studies

    Western blot

  • 内毒素水平< 1.000 Eu/µg
  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab84189 is purified using conventional chromatography techniques. Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • 形式Liquid
  • 补充说明Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 51 kDa FK506-binding protein
    • 52 kDa FK506 binding protein
    • 52 kDa FK506-binding protein
    • 52 kDa FKBP
    • 59 kDa immunophilin
    • FK506 binding protein 4
    • FK506-binding protein 4
    • FKBP 4
    • FKBP 52
    • FKBP 59
    • FKBP-4
    • FKBP-52
    • FKBP4
    • FKBP4_HUMAN
    • FKBP51
    • FKBP52 protein
    • FKBP59
    • HBI
    • Hsp 56
    • HSP binding immunophilin
    • HSP-binding immunophilin
    • Hsp56
    • Immunophilin FKBP52
    • N-terminally processed
    • p52
    • p59
    • p59 protein
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl-prolyl cis-trans isomerase FKBP4
    • Peptidylprolyl cis trans isomerase
    • PPIase
    • PPIase FKBP4
    • Rotamase
    • T cell FK506 binding protein 59kD
    see all
  • 功能Immunophilin protein with PPIase and co-chaperone activities (By similarity). Component of unliganded steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments (By similarity). The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.
  • 组织特异性Widely expressed.
  • 序列相似性Contains 2 PPIase FKBP-type domains.
    Contains 3 TPR repeats.
  • 结构域The PPIase activity is mainly due to the fisrt PPIase FKBP-type domain (1-138 AA).
    The C-terminal region (AA 375-458) is required to prevent tubulin polymerization.
    The chaperone activity resides in the C-terminal region, mainly between amino acids 264 and 400.
  • 翻译后修饰Phosphorylation by CK2 results in loss of HSP90 binding activity (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm > cytosol. Nucleus. Cytoplasm > cytoskeleton.
  • Information by UniProt

Recombinant human FKBP52 protein 图像

  • 15% SDS-PAGE showing ab84189 at approximately 53.9kDa (3µg).
  • Anti-FKBP52 antibody (ab97306) at 1/3000 dilution + Recombinant human FKBP52 protein (ab84189) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Recombinant human FKBP52 protein (ab84189)参考文献

ab84189 has not yet been referenced specifically in any publications.

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