The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific activity is > 300 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.
Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
Add 10 µl of recombinant FKBP1B protein with 1 µg in assay buffer.
Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
Record the increase in A405 nm for 30 minutes at 25°C.
% SDS-PAGE. ab113596 was purified using conventional chromatography.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00 Constituents: 0.32% Tris HCl, 20% Glycerol, 0.02% DTT
This product is an active protein and may elicit a biological response in vivo, handle with caution.
12.6 kDa FK506-binding protein
12.6 kDa FKBP
FK506 binding protein 1 like
FK506 binding protein 12.6
FK506 binding protein 1B
FK506 binding protein 1B 12.6 kDa
FK506-binding protein 1B
h FKBP 12
Peptidyl prolyl cis trans isomerase
Peptidyl prolyl cis trans isomerase 1B
Peptidyl-prolyl cis-trans isomerase FKBP1B
Associates with the ryanodine receptor (RYR-2) in cardiac muscle sarcoplasmic reticulum and may play a unique physiological role in excitation-contraction coupling in cardiac muscle. There are four molecules of FKBP12.6 per heart muscle RYR. Has the potential to contribute to the immunosuppressive and toxic effects of FK506 and rapamycin. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Isoform 1 and isoform 2 are Ubiquitous with highest levels in brain and thymus.
Belongs to the FKBP-type PPIase family. FKBP1 subfamily. Contains 1 PPIase FKBP-type domain.