Recombinant人FKBP14 protein (ab99328)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9NWM8
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHMALIPEPEVKIEVLQKPFICHRKTKGGDLM LVHYEGYLEKDGSLFHSTHKHNNGQPIWFTLGILEALKGWDQGLKGMCVG EKRKLIIPPALGYGKEGKGKIPPESTLIFNIDLLEIRNGPRSHESFQEMD LNDDWKLSKDEVKAYLKKEFEKHGAVVNESHHDALVEDIFDKEDEDKDGF ISAREFTYKHDEL
    • 分子量24 kDa including tags
    • 氨基酸20 to 211
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab99328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific activity is > 240 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1 µmole of suc-AAFP-pNA per minute at 25°C in Tris-Hcl pH8.0 using chymotrypsin.

    Activity assay

    1. Prepare 170 µl assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.
    2. Add 10 µl of recombinant FKBP14 protein with 1 µg in assay buffer.
    3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
    4. Add 20 µl pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
    5. Record the increase in A405 nm for 30 minutes at 25°C.
  • 应用

    Functional Studies

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab99328 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, PBS, pH 7.4

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 22 kDa FK506 binding protein
    • 22 kDa FK506-binding protein
    • 22 kDa FKBP
    • FK506 binding protein 14
    • FK506 binding protein 14 (22 kDa)
    • FK506-binding protein 14
    • FKB14_HUMAN
    • FKBP 22
    • FKBP-14
    • FKBP-22
    • FKBP14
    • FKBP22
    • FLJ20731
    • Peptidyl prolyl cis trans isomerase
    • Peptidyl-prolyl cis-trans isomerase FKBP14
    • PPIase
    • PPIase FKBP14
    • Rotamase
    see all
  • 功能PPIases accelerate the folding of proteins during protein synthesis.
  • 疾病相关Ehlers-Danlos syndrome, with progressive kyphoscoliosis, myopathy, and hearing loss
  • 序列相似性Contains 2 EF-hand domains.
    Contains 1 PPIase FKBP-type domain.
  • 细胞定位Endoplasmic reticulum lumen.
  • Information by UniProt

Recombinant human FKBP14 protein 图像

  • 15% SDS-PAGE analysis of 3µg ab99328.

Recombinant human FKBP14 protein (ab99328)参考文献

ab99328 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"