Recombinant人Fibroblast activation protein, alpha (ab79623)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 氨基酸29 to 760
    • 标签His tag C-Terminus

技术指标

Our Abpromise guarantee covers the use of ab79623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 10.2 pmol/min/µg. Assay Condition: 50 mM Tris-HCl (pH7.5), 10 mM MgCl2, 1 mM MnCl2, 5µM substrate (A-P-AMC), room temperature.
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Preservative: 1.36% Imidazole
    Constituents: 0.64% Tris HCl, 20% Glycerol, 0.64% Sodium chloride, 0.02% Potassium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • 170 kDa melanoma membrane bound gelatinase
    • 170 kDa melanoma membrane-bound gelatinase
    • DPPIV
    • FAP
    • FAPA
    • Fibroblast activation protein alpha
    • Integral membrane serine protease
    • SEPR_HUMAN
    • Seprase
    see all
  • 功能In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM. May have a role in tissue remodeling during development and wound healing, and may contribute to invasiveness in malignant cancers.
  • 组织特异性Fibroblast specific.
  • 序列相似性Belongs to the peptidase S9B family.
  • 翻译后修饰N-glycosylated.
    The N-terminus may be blocked.
  • 细胞定位Cell membrane. Cell projection > lamellipodium membrane. Cell projection > invadopodium membrane. Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma.
  • Information by UniProt

Recombinant human Fibroblast activation protein, alpha 图像

  • Image showing specific activity of ab79623.
  • SDS-PAGE showing 3ug ab79623 at approximately 85.5kDa.

Recombinant human Fibroblast activation protein, alpha (ab79623)参考文献

ab79623 has not yet been referenced specifically in any publications.

Product Wall



The Active human Fibroblast activation protein, alpha protein fragment (ab79623) contains a His tag at the C terminal end.

Thank you for your enquiry and interest in our products.

I would suggest loading between 1-5 ug per lane but this may need to be optimized since it depends on the detection system you will apply. Please take a look at the SDS-PAGE image on th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"