Recombinant人Fibrillarin protein (ab99443)

概述

  • 产品名称Recombinant人Fibrillarin protein
  • 蛋白长度Protein fragment

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP22087
    • 种属Human
    • 序列MGSSHHHHHHSSGLVPRGSHRSMGKNVMVEPHRHEGVFICRGKEDALVTK NLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKP GAKVLYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTN IIPVIEDARHPHKYRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGH FVISIKANCIDSTASAEAVFASEVKKMQQENMKPQEQLTLEPYERDHAVV VGVYRPPPKVKN
    • 分子量29 kDa including tags
    • 氨基酸83 to 321
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab99443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 质谱法
    MALDI-TOF
  • 纯度>= 85 % SDS-PAGE.
    ab99443 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 0.2M Sodium chloride, 20mM Tris HCl, 1mM EDTA, 1mM DTT, pH 8.0

常规信息

  • 别名
    • 34 kD nucleolar scleroderma antigen
    • 34 kDa nucleolar scleroderma antigen
    • Fbl
    • FBRL_HUMAN
    • FIB
    • FIB1
    • FLRN
    • Histone-glutamine methyltransferase
    • Nop1p
    • RNA U3 small nucleolar interacting protein 1
    • RNU3IP1
    • rRNA 2' O methyltransferase fibrillarin
    • rRNA 2'-O-methyltransferase fibrillarin
    see all
  • 功能S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ104me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239).
  • 序列相似性Belongs to the methyltransferase superfamily. Fibrillarin family.
  • 翻译后修饰By homology to other fibrillarins, some or all of the N-terminal domain arginines are modified to asymmetric dimethylarginine (DMA).
  • 细胞定位Nucleus, nucleolus. Fibrillar region of the nucleolus.
  • Information by UniProt

Recombinant Human Fibrillarin protein 图像

  • 15% SDS-PAGE showing ab99443 at approximately 28.9kDa (3µg).

Recombinant Human Fibrillarin protein (ab99443)参考文献

ab99443 has not yet been referenced specifically in any publications.

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