The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
< 0.100 Eu/µg
% SDS-PAGE. ab108556 is 0.2 µm filtered.
Working aliquots are stable for up to 3 months when stored at -20°C.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
IgG1: Secreted. Cell membrane; Single-pass membrane protein
Functional Studies - FGF21 protein (ab108556)
ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF
21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.
Lane 1: 1 µg/ml hFGF21-His (competitor)
Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
Lane 3: 2.2 µg/ml mCD137-Fc
Lane 4: 100 ng/µl FGF-b
Lane 5: control (non-treated)
has not yet been referenced specifically in any publications.