Recombinant人FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)

概述

  • 产品名称Recombinant人FGF21 + IgG1 fusion protein (Fc Chimera Active)
  • 蛋白长度Full length protein
  • 描述
    Recombinant人FGF21 + IgG1 protein (Fc Chimera Active)

描述

  • 性质Recombinant
  • 来源HEK 293 cells
  • 氨基酸序列
    • AccessionQ9NSA1
    • 种属Human
    • 分子量50 kDa including tags
    • 额外的序列信息Human FGF21 (aa 1-209) is fused at the C-terminus to the Fc portion of human IgG1.

技术指标

Our Abpromise guarantee covers the use of ab108556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activates ERK and FRS2alpha phosphorylation in bKlotho expressing cells.
  • 应用

    Functional Studies

    SDS-PAGE

  • 内毒素水平< 0.100 Eu/µg
  • 纯度>= 95 % SDS-PAGE.
    ab108556 is 0.2 µm filtered.
  • 形式Liquid
  • 补充说明Working aliquots are stable for up to 3 months when stored at -20°C.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 细胞定位FGF21: Secreted. IgG1: Secreted. Cell membrane; Single-pass membrane protein

Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) 图像

  • ERK and FRS2alpha phosphorylation induced by FGF21 in bKlotho expressing cells.
    bKlotho expressing cells were serum starved for 16hr and then stimulated with hFGF21-His (competitor), ab108556 (FGF 21-Fc), mCD137-Fc (Fc control) and FGF-b (positive control) for 10 minutes, respectively.
    Antibodies against pFRS2alpha, pERK1/2 & total ERK1/2 were used for immunoblotting.

    Lane 1: 1 µg/ml hFGF21-His (competitor)
    Lane 2: 2.2 µg/ml ab108556 (hFGF21-Fc)
    Lane 3: 2.2 µg/ml mCD137-Fc
    Lane 4: 100 ng/µl FGF-b
    Lane 5: control (non-treated)

Recombinant human FGF21 + IgG1 fusion protein (Fc Chimera Active) (ab108556)参考文献

ab108556 has not yet been referenced specifically in any publications.

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