Recombinant人FGF2 protein (ab88394)

概述

描述

  • 性质
    Recombinant
  • 来源
    HEK 293 cells
  • 氨基酸序列
    • 种属
      Human
    • 序列
      MAAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVD GVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVT DE CFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAI LFLP MSAKS

技术指标

Our Abpromise guarantee covers the use of ab88394 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性
    Activity: The ED50 of ab88394 is typically 0.05-0.1 ng/ml as measured in a cell proliferation assay using human umbilical endothelial cells (HUVECs).
  • 应用

    SDS-PAGE

    Functional Studies

  • 纯度
    > 95 % SDS-PAGE.

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Trehalose, 1% Human serum albumin

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • 复溶
    It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

常规信息

  • 别名
    • Basic fibroblast growth factor
    • Basic fibroblast growth factor bFGF
    • BFGF
    • FGF 2
    • FGF B
    • FGF-2
    • Fgf2
    • FGF2 basic
    • FGF2_HUMAN
    • FGFB
    • Fibroblast growth factor 2
    • Fibroblast growth factor 2 (basic)
    • Fibroblast growth factor, basic
    • HBGF 2
    • HBGF-2
    • HBGF2
    • HBGH 2
    • HBGH2
    • Heparin binding growth factor 2 precursor
    • Heparin-binding growth factor 2
    • Prostatropin
    see all
  • 功能
    Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107).
  • 组织特异性
    Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue.
  • 序列相似性
    Belongs to the heparin-binding growth factors family.
  • 翻译后修饰
    Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
    Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing.
  • 细胞定位
    Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57.
  • Information by UniProt

Recombinant human FGF2 protein 图像

  • 1D SDS-PAGE of ab88394 before and after treatment with glycosidases to remove oligosaccharides.
    Lane 1: ab88394
    Lane 2: ab88394 treated with PNGase F to remove potential N-linked glycans
    Lane 3: ab88394 treated with a glycosidase cocktail to remove potential N- and O-linked glycans.

    Additional bands in lane 2 and lane 3 are glycosidase enzymes. The results suggest that ab88394 is not glycosylated.

Recombinant human FGF2 protein (ab88394)参考文献

ab88394 has not yet been referenced specifically in any publications.

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