重组人FGF1蛋白(Active) (ab155608)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人FGF1蛋白(Active)
参阅全部 FGF1 蛋白酶 -
生物活性
Measured by its binding ability in a BLI assay. Loaded ab168696 on HIS1K Biosensor, can bind ab155608 with an affinity constant of 0.16 μM as determined in BLI assay (ForteBio Octet Red96e).
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纯度
> 98 % SDS-PAGE. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAES VGEVYIKSTE TGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVG LKKNGSCKRG PRTHYGQKAILFLPLPVSSD -
预测分子量
16 kDa -
氨基酸
16 to 155 -
额外的序列信息
This product is the mature full length protein from aa 16 to 155. The propeptide is not included.
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技术指标
Our Abpromise guarantee covers the use of ab155608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Lyophilized -
补充说明
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 95% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 200 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
常规信息
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别名
- Acidic fibroblast growth factor
- aFGF
- Beta endothelial cell growth factor
see all -
功能
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
序列相似性
Belongs to the heparin-binding growth factors family. -
细胞定位
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
图片
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Loaded ab168696 on HIS1K Biosensor, can bind ab155608 with an affinity constant of 0.16 μM as determined in BLI assay (ForteBio Octet Red96e).
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SDS-PAGE of reduced ab155608 stained overnight with Coomassie Blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab155608 尚未被引用在任何文献中。