Recombinant人Estrogen Receptor alpha protein (ab82606)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • 种属Human
    • 序列MTMTLHTKAS GMALLHQIQG NELEPLNRPQ LKIPLERPLG EVYLDSSKPA VYNYPEGAAY EFNAAAAANA QVYGQTGLPY GPGSEAAAFG SNGLGGFPPL NSVSPSPLML LHPPPQLSPF LQPHGQQVPY YLENEPSGYT VREAGPPAFY RPNSDNRRQG GRERLASTND KGSMAMESAK ETRYCAVCND YASGYHYGVW SCEGCKAFFK RSIQGHNDYM CPATNQCTID KNRRKSCQAC RLRKCYEVGM MKGGIRKDRR GGRMLKHKRQ RDDGEGRGEV GSAGDMRAAN LWPSPLMIKR SKKNSLALSL TADQMVSALL DAEPPILYSE YDPTRPFSEA SMMGLLTNLA DRELVHMINW AKRVPGFVDL TLHDQVHLLE CAWLEILMIG LVWRSMEHPG KLLFAPNLLL DRNQGKCVEG MVEIFDMLLA TSSRFRMMNL QGEEFVCLKS IILLNSGVYT FLSSTLKSLE EKDHIHRVLD KITDTLIHLM AKAGLTLQQQ HQRLAQLLLI LSHIRHMSNK GMEHLYSMKC KNVVPLYDLL LEMLDAHRLH APTSRGGASV EETDQSHLAT AGSTSSHSLQ KYYITGEAEG FPATV
    • 分子量68 kDa
    • 氨基酸1 to 595

技术指标

Our Abpromise guarantee covers the use of ab82606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Other

    Western blot

  • 纯度> 90 % SDS-PAGE.
    ab82606 is greater than 90% homogeneous and based on SDS-PAGE analysis, purified by affinity and FPLC chromatography.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

常规信息

  • 别名
    • DKFZp686N23123
    • ER
    • ER alpha
    • ER-alpha
    • Era
    • ESR
    • ESR1
    • ESR1_HUMAN
    • ESRA
    • Estradiol receptor
    • Estrogen nuclear receptor alpha
    • Estrogen receptor
    • Estrogen receptor 1
    • Estrogen receptor alpha
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform
    • Estrogen receptor alpha delta 4 +49 isoform
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform
    • Estrogen receptor alpha E1 E2 1 2
    • Estrogen receptor alpha E1 N2 E2 1 2
    • ESTRR
    • NR3A1
    • Nuclear receptor subfamily 3 group A member 1
    see all
  • 功能Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.
  • 序列相似性Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 翻译后修饰Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).
  • 细胞定位Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt

Recombinant Human Estrogen Receptor alpha protein 图像

  • Anti-Estrogen Receptor alpha antibody (ab37438) at 1 µg/ml + Recombinant Human Estrogen Receptor alpha protein (ab82606) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human Estrogen Receptor alpha protein (ab82606)参考文献

ab82606 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your reply.

I am happy to confirm that ab82606 has a 6x HIS tag at the N-terminus.

I hope this information is helpful for your customer and wish them good luck with their research.

Thank you for contacting us and sorry for the delay in getting back to you.

We have no record of the estrogen receptor alpha (ab82606) and progesterone receptor (ab75958) proteins being used in the assays you have described. The estrogen rece...

Read More

Thank you for contacting Abcam. For your first question, 1 unit is equal to 1 nanogram of purified protein. As to Abcam protocols for protein-protein interaction, we do not have our own protocols but I did find the following link that may be useful: ht...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"