Recombinant人ERp57 protein (ab92937)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KTVAYTEQKM TSGKIKKFIQ ENIFGICPHM TEDNKDLIQG KDLLIAYYDV DYEKNAKGSN YWRNRVMMVA KKFLDAGHKL NFAVASRKTF SHELSDFGLE STAGEIPVVA IRTAKGEKFV MQEEFSRDGK ALERFLQDYF DGNLKRYLKS EPIPESNDGP VKVVVAENFD EIVNNENKDV LIEFYAPWCG HCKNLEPKYK ELGEKLSKDP NIVIAKMDAT ANDVPSPYEV RGFPTIYFSP ANKKLNPKKY EGGRELSDFI SYLQREATNP PVIQEEKPKK KKKAQEDL
    • 分子量
      59 kDa
    • 氨基酸
      25 to 505
    • 标签
      His tag N-Terminus
    • 额外的序列信息
      Full length mature protein minus the signal peptide.

技术指标

Our Abpromise guarantee covers the use of ab92937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab92937 is purified using conventional chromatography techniques.
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 10% Glycerol, 0.1M Sodium chloride, 20mM Tris HCl, 1mM DTT, pH 8.0

常规信息

  • 别名
    • 58 kDa glucose regulated protein
    • 58 kDa glucose-regulated protein
    • 58 kDa microsomal protein
    • Disulfide isomerase ER 60
    • Disulfide isomerase ER-60
    • Endoplasmic reticulum resident protein 57
    • Endoplasmic reticulum resident protein 60
    • ER p57
    • ER protein 57
    • ER protein 60
    • ERp 57
    • ERp57
    • ERp60
    • ERp61
    • Glucose Regulated Protein 58 Kd
    • GRP 57
    • GRP 58
    • GRP57
    • HsT17083
    • p58
    • PDIA 3
    • PDIA3
    • PDIA3_HUMAN
    • Phospholipase C alpha
    • PI PLC
    • Protein disulfide isomerase A3
    • Protein disulfide isomerase family A member 3
    • Protein disulfide-isomerase A3
    see all
  • 组织特异性
    Detected in the flagellum and head region of spermatozoa (at protein level).
  • 序列相似性
    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 细胞定位
    Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt

图片

  • 15% SDS-PAGE showing ab92937 (3µg) at approximately 58.5kDa.

文献

ab92937 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Application
Functional Studies
We ordered the recombinant ERp57 for use in an experiment to examine the catalytic activity of the enzyme. Using the product provided by abcam, we were able to successful observe the catalytic reduction of insulin. Thus in our experience, this product can be used for functional studies.
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Abcam user community

Verified customer

提交于 Apr 07 2014

Vielen Dank für Ihre Anfrage.

Leider haben wir die Aktivität dieses Produktes nicht getestet.

Da es allerdings mit 10% Glycerin und DTT versetzt wurde, gehe ich eher davon aus, dass dieses Protein nicht mehr aktiv ist.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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