Recombinant人ERO1L protein (ab125981)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 分子量
      18 kDa
    • 氨基酸
      37 to 189
    • 标签
      His-DHFR tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab125981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at -20ºC.

    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride

  • 复溶
    Reconstitute with water to desired concentration.

常规信息

  • 别名
    • Endoplasmic oxidoreductin 1 like protein
    • Endoplasmic oxidoreductin-1-like protein
    • Endoplasmic reticulum oxidoreductase 1 alpha
    • Endoplasmic reticulum oxidoreductin 1-like
    • ERO1 alpha
    • ERO1 L
    • ERO1 Lalpha
    • ERO1 like protein alpha
    • ERO1-alpha
    • ERO1-L
    • ERO1-L-alpha
    • ERO1-like (S. cerevisiae)
    • ERO1-like alpha
    • ERO1-like protein alpha
    • ERO1-like, S. cerevisiae, homolog of, alpha
    • ERO1A
    • ERO1A_HUMAN
    • ERO1L
    • ERO1LA
    • Oxidoreductin 1 Lalpha
    • Oxidoreductin-1-L-alpha
    • PRO865
    • UNQ434
    see all
  • 功能
    Essential oxidoreductase that oxidizes proteins in the endoplasmic reticulum to produce disulfide bonds. Acts by oxidizing directly P4HB/PDI isomerase through a direct disulfide exchange. Does not act as a direct oxidant of folding substrate, but relies on P4HB/PDI to transfer oxidizing equivalent. Associates with ERP44 but not with GRP54, demonstrating that it does not oxidize all PDI related proteins and can discriminate between PDI and related proteins. Its reoxidation probably involves electron transfer to molecular oxygen via FAD. Acts independently of glutathione. May be responsible for a significant proportion of reactive oxygen species (ROS) in the cell, thereby being a source of oxidative stress. Required for the folding of immunoglobulin proteins. Responsible for the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin.
  • 组织特异性
    Widely expressed at low level. Expressed at high level in upper digestive tract. Highly expressed in esophagus. Weakly expressed in stomach and duodenum.
  • 序列相似性
    Belongs to the EROs family.
  • 翻译后修饰
    N-glycosylated.
    The Cys-94/Cys-99 and Cys-394/Cys-397 disulfide bonds constitute the redox-active center. The Cys-94/Cys-99 disulfide bond may accept electron from P4HB and funnel them to the active site disulfide Cys-394/Cys-397.
  • 细胞定位
    Endoplasmic reticulum membrane. The association with ERP44 is essential for its retention in the endoplasmic reticulum.
  • Information by UniProt

文献

ab125981 has not yet been referenced specifically in any publications.

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