Recombinant人ERK1 protein (ab43623) (ab43623)

概述

  • 产品名称
    Recombinant人ERK1 protein (ab43623)
    参阅全部 ERK1 蛋白酶
  • 蛋白长度
    Full length protein
  • 描述
    Recombinant人ERK1 protein

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human

技术指标

Our Abpromise guarantee covers the use of ab43623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Western blot

  • 形式
    Liquid
  • 补充说明

    ab43623 (Human ERK1 full length protein) can be utilized as a substrate for the following active protein kinases:

     

    ab85299 (Active human MEKK2 full length protein)

    ab55700 (Active human MAP3K8 protein fragment)

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

常规信息

  • 别名
    • ERK
    • ERK 1
    • ERK-1
    • ERK1
    • ERT 2
    • ERT2
    • Extracellular Signal Regulated Kinase 1
    • Extracellular signal related kinase 1
    • Extracellular signal-regulated kinase 1
    • HGNC6877
    • HS44KDAP
    • HUMKER1A
    • Insulin Stimulated MAP2 Kinase
    • Insulin-stimulated MAP2 kinase
    • MAP Kinase
    • MAP kinase 1
    • MAP kinase 3
    • MAP kinase isoform p44
    • MAPK
    • MAPK 1
    • MAPK 3
    • MAPK1
    • Mapk3
    • MGC20180
    • Microtubule Associated Protein 2 Kinase
    • Microtubule-associated protein 2 kinase
    • Mitogen Activated Protein Kinase 3
    • Mitogen-activated protein kinase 1
    • Mitogen-activated protein kinase 3
    • MK03_HUMAN
    • OTTHUMP00000174538
    • OTTHUMP00000174541
    • p44 ERK1
    • p44 MAPK
    • p44-ERK1
    • p44-MAPK
    • P44ERK1
    • P44MAPK
    • PRKM 3
    • PRKM3
    • Protein Kinase Mitogen Activated 3
    see all
  • 功能
    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
  • 序列相似性
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • 结构域
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻译后修饰
    Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
  • Information by UniProt

Recombinant Human ERK1 protein (ab43623) 图像

  • Anti-ERK1 antibody [M233] (ab88480) at 1/1000 dilution + Recombinant Human ERK1 protein (ab43623) (ab43623) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

Recombinant Human ERK1 protein (ab43623) (ab43623)参考文献

ab43623 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab43623.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册