The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Determined by the dose-dependent stimulation of the proliferation of mouse Balb/3T3 cells. The expected ED50 for this effect is 150-300ng/ml corresponding to a specific activity of 3,334-6,667IU/mg.
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Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).