Recombinant人Eph receptor B4 protein (ab42608)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 序列attggaaa ctgctgatct gaagtgggtg acattccctc aggtggacgg gcagtgggag gaactgagcg gcctggatga ggaacagcac agcgtgcgca cctacgaagt gtgtgacgtg cagcgtgccc cgggccaggc ccactggctt cgcacaggtt gggtcccacg gcggggcgcc gtccacgtgt acgccacgct gcgcttcacc atgctcgagt gcctgtccct gcctcgggct gggcgctcct gcaaggagac cttcaccgtc ttctactatg agagcgatgc ggacacggcc acggccctca cgccagcctg gatggagaac ccctacatca aggtggacac ggtggccgcg gagcatctca cccggaagcg ccctggggcc gaggccaccg ggaaggtgaa tgtcaagacg ctgcgtc
    • 氨基酸563 to 987

技术指标

Our Abpromise guarantee covers the use of ab42608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Specific Activity: 125 U/mg. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30?C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 2 ug /ml recombinant EPHB4.
  • 应用

    Functional Studies

  • 纯度> 90 % SDS-PAGE.

  • 形式Liquid
  • 补充说明This protein was expressed in a Baculovirus infected Sf9 cell expression system.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Ephb4
    • EPHB4_HUMAN
    • Ephrin receptor EphB4
    • Ephrin type-B receptor 4
    • Hepatoma Transmembrane Kinase
    • HTK
    • MYK 1
    • MYK1
    • TYRO 11
    • TYRO11
    • Tyrosine-protein kinase receptor HTK
    • Tyrosine-protein kinase TYRO11
    see all
  • 功能Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • 组织特异性Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human Eph receptor B4 protein (ab42608)参考文献

ab42608 has not yet been referenced specifically in any publications.

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