Recombinant人Eph receptor B2 protein (ab42607)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected Sf9 cells
  • 氨基酸序列
    • 种属Human
    • 序列F ERADSEYTDK LQHYTSGHMT PGMKIYIDPF TYEDPNEAVR EFAKEIDISC VKIEQVIGAG EFGEVCSGHL KLPGKREIFV AIKTLKSGYT EKQRRDFLSE ASIMGQFDHP NVIHLEGVVT KSTPVMIITE FMENGSLDSF LRQNDGQFTV IQLVGMLRGI AAGMKYLADM NYVHRDLAAR NILVNSNLVC KVSDFGLSRF LEDDTSDPTY TSALGGKIPI RWTAPEAIQY RKFTSASDVW SYGIVMWEVM SYGERPYWDM TNQDVINAIE QDYRLPPPMD CPSALHQLML DCWQKDRNHR PKFGQIVNTL DKMIRNPNSL KAMAPLSSGI NLPLLDRTIP DYTSFNTVDE WLEAIKMGQY KESFANAGFT SFDVVSQMMM EDILRVGVTL AGHQKKILNS IQVMRAQMNQ IQSVEGQPLA RRPRATGRTK RCQPRDVTKK TCNSNDGKKK GMGKKKTDPG RGREIQGIFF KEDSHKESND CSCGG
    • 氨基酸569 to 1055

技术指标

Our Abpromise guarantee covers the use of ab42607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: 150 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of tyrosine substrate per minute at pH 7.4 and 30C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.1 mM ATP, 30 ug/ml Poly (Glu:Tyr)4:1 substrate, and 4 ug /ml recombinant EPHB2.
  • 应用

    Inhibition Assay

  • 形式Liquid
  • 补充说明Expressed in a Baculovirus infected Sf9 expression system. Expected molecular weight of 52 kDa.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, 0.05% Tween 20, 3mM DTT, 25mM Tris HCl, 100mM Sodium chloride, pH 8.0

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • cek5
    • Developmentally regulated EPH related tyrosine kinase
    • DRT
    • EK5
    • ELK related protein tyrosine kinase
    • Eph receptor B2
    • EPH tyrosine kinase 3
    • EPH-like kinase 5
    • EPHB2
    • EPHB2_HUMAN
    • Ephrin type B receptor 2
    • Ephrin type-B receptor 2
    • EPHT 3
    • ERK
    • ETECK
    • hEK5
    • Nuk
    • Prkm 5
    • Receptor protein tyrosine kinase HEK 5
    • Renal carcinoma antigen NY-REN-47
    • Sek 3
    • Tyro 5
    • Tyrosine protein kinase receptor CEK 5
    • Tyrosine protein kinase receptor EPH 3
    • Tyrosine protein kinase receptor QEK 5
    • Tyrosine-protein kinase receptor EPH-3
    • Tyrosine-protein kinase TYRO5
    see all
  • 功能Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
  • 组织特异性Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
  • 疾病相关Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human Eph receptor B2 protein (ab42607)参考文献

ab42607 has not yet been referenced specifically in any publications.

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