Recombinant人Eph receptor A2 protein (ab114440)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP29317
    • 种属Human
    • 序列LLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEW LVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEG ATSCECEEGFFRAPQDPASMPCT
    • 分子量39 kDa including tags
    • 氨基酸204 to 326

技术指标

Our Abpromise guarantee covers the use of ab114440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    Western blot

    SDS-PAGE

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best use within three months from the date of receipt of this protein.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • ARCC2
    • AW545284
    • CTPA
    • CTPP1
    • CTRCT6
    • EC 2.7.10.1
    • Eck
    • Eph receptor A2
    • EPHA2
    • EPHA2_HUMAN
    • Ephrin receptor
    • Ephrin receptor EphA2
    • Ephrin type A receptor 2
    • Ephrin type-A receptor 2
    • Epithelial cell kinase
    • Epithelial cell receptor protein tyrosine kinase
    • Myk 2
    • Myk2
    • Sek 2
    • Sek2
    • Soluble EPHA2 variant 1
    • Tyrosine protein kinase receptor ECK
    • Tyrosine-protein kinase receptor ECK
    • Tyrosine-protein kinase receptor MPK-5
    • Tyrosine-protein kinase receptor SEK-2
    see all
  • 功能Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • 组织特异性Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • 疾病相关Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant Human Eph receptor A2 protein 图像

  • ab114440 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Recombinant Human Eph receptor A2 protein (ab114440)参考文献

ab114440 has not yet been referenced specifically in any publications.

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