Recombinant人Eph receptor A1 protein (ab101778)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus
  • 氨基酸序列
    • AccessionP21709
    • 种属Human
    • 分子量72 kDa including tags
    • 氨基酸569 to 976

技术指标

Our Abpromise guarantee covers the use of ab101778 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性The Specific activity of ab101778 was determined to be 28 nmol/min/mg.
  • 应用

    Western blot

    Functional Studies

  • 纯度> 90 % Densitometry.
    Purity was determined to be >90% by densitometry.
  • 形式Liquid
  • 补充说明

    ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • EPH
    • EPH receptor A1
    • EPH tyrosine kinase
    • EPH tyrosine kinase 1
    • Epha1
    • EPHA1_HUMAN
    • Ephrin receptor Eph A1
    • Ephrin type A receptor 1
    • Ephrin type-A receptor 1
    • EPHT
    • EPHT 1
    • EPHT1
    • Erythropoietin producing hepatoma amplified sequence
    • Erythropoietin-producing hepatoma receptor
    • Esk
    • hEpha1
    • MGC163163
    • Oncogene EPH
    • Soluble EPHA1 variant 1
    • Soluble EPHA1 variant 2
    • Tyrosine protein kinase receptor EPH
    • Tyrosine-protein kinase receptor EPH
    see all
  • 功能Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.
  • 组织特异性Overexpressed in several carcinomas.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 1 Eph LBD (Eph ligand-binding) domain.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • 翻译后修饰Phosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
    Ubiquitinated.
  • 细胞定位Cell membrane.
  • Information by UniProt

Recombinant human Eph receptor A1 protein 图像

  • SDS-PAGE showing ab101778 at approximately 72kDa.
  • The Specific activity of ab101778 was determined to be 28 nmol/min/mg.

Recombinant human Eph receptor A1 protein (ab101778)参考文献

ab101778 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab101778.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"