重组人eNOS蛋白(Tagged) (ab198066)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 71% SDS-PAGE
- Tags: His-DDDDK tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组人eNOS蛋白(Tagged)
参阅全部 eNOS 蛋白酶 -
纯度
>= 71 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Human -
序列
MHHHHHHDYKDDDDKGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAP EPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSA QAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYS SIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVG RIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCP GRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRF DVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAV SNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLD TRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQK ARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAK GTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQ QLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESF AAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNT DSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGD ELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYR LSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTG GQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGS PGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPRE QQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQ PRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPG DPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKG LQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKT YVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGD MELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLR GAVPWAFDPPGSDTNSP -
预测分子量
135 kDa including tags -
氨基酸
2 to 1203 -
标签
His-DDDDK tag N-Terminus -
额外的序列信息
This product is for the mature full length protein from aa 2 to 1203. The initial Met is not included. GenBank Accession No. NM_000603.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab198066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.77% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)
常规信息
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别名
- cNOS
- Constitutive NOS
- EC NOS
see all -
功能
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. -
组织特异性
Platelets, placenta, liver and kidney. -
疾病相关
Variation in NOS3 seem to be associated with susceptibility to coronary spasm. -
序列相似性
Belongs to the NOS family.
Contains 1 FAD-binding FR-type domain.
Contains 1 flavodoxin-like domain. -
翻译后修饰
Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity. -
细胞定位
Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle and which is favored by interaction with NOSIP and results in a reduced enzymatic activity. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab198066 尚未被引用在任何文献中。