Recombinant人ELK1 protein (ab73451)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • 种属
      Human
    • 分子量
      60 kDa
    • 氨基酸
      307 to 428
    • 额外的序列信息
      ELK-1 (amino acids 307-428) Human Recombinant is produced in E.Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL).

技术指标

Our Abpromise guarantee covers the use of ab73451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

    SDS-PAGE

  • 纯度
    > 95 % SDS-PAGE.
    ab73451 is purified by proprietary chromatographic techniques.
  • 形式
    Lyophilised
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Preservative: None

  • 复溶
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute lyophilized in distilled water to a concentration of 0.2-1.0 mg/mL.

常规信息

  • 别名
    • ELK 1
    • Elk1
    • ELK1 member of ETS oncogene family
    • ELK1 protein
    • ELK1, ETS transcription factor
    • ELK1_HUMAN
    • ELK2 member of ETS oncogene family
    • ETS domain containing protein Elk 1
    • ETS domain containing protein Elk1
    • ETS domain protein Elk1
    • ETS domain-containing protein Elk-1
    • ETS like gene 1
    • Member of ETS oncogene family
    • Oncogene Elk1
    • Tyrosine kinase (ELK1) oncogene
    see all
  • 功能
    Stimulates transcription. Binds to purine-rich DNA sequences. Can form a ternary complex with the serum response factor and the ETS and SRF motifs of the fos serum response element.
  • 组织特异性
    Lung and testis.
  • 序列相似性
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • 翻译后修饰
    Sumoylation represses transcriptional activator activity as it results in recruitment of HDAC2 to target gene promoters which leads to decreased histone acetylation and reduced transactivator activity. It also regulates nuclear retention.
    On mitogenic stimulation, phosphorylated on C-terminal serine and threonine residues by MAPK1. Ser-383 and Ser-389 are the preferred sites for MAPK1. In vitro, phosphorylation by MAPK1 potentiates ternary complex formation with the serum responses factors, SRE and SRF. Phosphorylation leads to loss of sumoylation and restores transcriptional activator activity.
  • 细胞定位
    Nucleus.
  • Information by UniProt

文献

ab73451 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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