Recombinant人eIF4A3 protein (ab91993)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human
    • 序列DVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGL LALGDYMNVQC HACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRR SLRTRAIKMLVLDEADEMLNKGFK EQIYDVYRYLPPATQVVLISATLP HEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVE REEWKFDTLCD LYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIM KEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRS GRYGRKGVAINFV KNDDIRILRDIEQYYSTQIDEMPMNVADLI
    • 氨基酸77 to 411

技术指标

Our Abpromise guarantee covers the use of ab91993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Mass Spectrometry

  • 形式Lyophilised
  • 补充说明Protein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at 4°C prior to reconstitution. Store at -80°C.

    Preservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5

  • 复溶Reconstitute with 155 µl aqua dest.

常规信息

  • 别名
    • ATP-dependent RNA helicase DDX48
    • ATP-dependent RNA helicase eIF4A-3
    • DDX48
    • DEAD box protein 48
    • eIF-4A-III
    • eIF4A-III
    • EIF4A3
    • eIF4AIII
    • Eukaryotic initiation factor 4A-III
    • Eukaryotic initiation factor 4A-like NUK-34
    • Eukaryotic translation initiation factor 4A isoform 3
    • hNMP 265
    • IF4A3_HUMAN
    • NMP 265
    • NMP265
    • Nuclear matrix protein 265
    • NUK34
    see all
  • 功能ATP-dependent RNA helicase. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed.
  • 组织特异性Ubiquitously expressed.
  • 序列相似性Belongs to the DEAD box helicase family. eIF4A subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 细胞定位Nucleus. Nucleus speckle. Cytoplasm. Nucleocytoplasmic shuttling protein. Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Detected in dendritic layer as well as the nuclear and cytoplasmic (somatic) compartments of neurons. Colocalizes with STAU1 and FMR1 in dendrites.
  • Information by UniProt

Recombinant Human eIF4A3 protein 图像

  • The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

Recombinant Human eIF4A3 protein (ab91993)参考文献

ab91993 has not yet been referenced specifically in any publications.

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