Recombinant人eIF3e protein (ab114770)

概述

描述

  • 性质Recombinant
  • 来源Wheat germ
  • 氨基酸序列
    • AccessionP60228
    • 种属Human
    • 序列RIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGN NAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY
    • 分子量37 kDa including tags
    • 氨基酸346 to 445

技术指标

Our Abpromise guarantee covers the use of ab114770 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Western blot

    ELISA

  • 形式Liquid
  • 补充说明Protein concentration is above or equal to 0.05 mg/ml.
    Best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • eIF-3 p48
    • eIF3e
    • EIF3E_HUMAN
    • EIF3S6
    • eIFe
    • Eukaryotic translation initiation factor 3 subunit 6
    • Eukaryotic translation initiation factor 3 subunit E
    • eukaryotic translation initiation factor 3, subunit 6 (48kD)
    • INT6
    • mammary tumor-associated protein INT6
    • murine mammary tumor integration site 6 (oncogene homolog)
    • Viral integration site protein INT-6 homolog
    see all
  • 功能Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
  • 组织特异性Ubiquitously expressed. Expressed at highest levels in appendix, lymph, pancreas, skeletal muscle, spleen and thymus.
  • 序列相似性Belongs to the eIF-3 subunit E family.
    Contains 1 PCI domain.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Cytoplasm. Nucleus > PML body.
  • Information by UniProt

Recombinant Human eIF3e protein 图像

  • ab114770 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.

Recombinant Human eIF3e protein (ab114770)参考文献

ab114770 has not yet been referenced specifically in any publications.

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