Recombinant人EIF2S1 protein (ab123468)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionP05198
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ENMPIKINLI APPRYVMTTT TLERTEGLSV LSQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQM ERLERENAEV DGDDDAEEME AKAED
    • 分子量38 kDa including tags
    • 氨基酸1 to 315
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab123468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

  • 纯度> 85 % SDS-PAGE.
    ab123468 was purified by proprietary chromatographic techniques and filter sterilized. ab123468 is greater than 85% as determined by SDS-PAGE.
  • 形式Liquid
  • 补充说明Although stable at 4C for 1 week, ab123468 should be stored desiccated below -18C. Please prevent freeze thaw cycles.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Please see notes section.

    pH: 8.00
    Constituents: 0.32% Tris HCl, 10% Glycerol, 0.58% Sodium chloride

常规信息

  • 别名
    • EIF 2
    • EIF 2 alpha
    • EIF 2A
    • EIF 2alpha
    • eIF-2-alpha
    • eIF-2A
    • EIF-2alpha
    • EIF2
    • EIF2 alpha
    • EIF2A
    • EIF2S1
    • Eukaryotic translation initiation factor 2 subunit 1
    • Eukaryotic translation initiation factor 2 subunit 1 alpha
    • Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa
    • Eukaryotic translation initiation factor 2 subunit alpha
    • IF2A_HUMAN
    see all
  • 功能Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
  • 序列相似性Belongs to the eIF-2-alpha family.
    Contains 1 S1 motif domain.
  • 翻译后修饰Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.
  • 细胞定位Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).
  • Information by UniProt

Recombinant Human EIF2S1 protein (ab123468)参考文献

ab123468 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123468.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"