重组人EHMT2/G9A蛋白(ab80323)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组人EHMT2/G9A蛋白
参阅全部 EHMT2/G9A 蛋白酶 -
生物活性
Activity: 22 pmol/min/µg. Assay conditions: Enzyme was incubated with 0.5 µM of biotinylated H3 peptide with 40 µM AdoMet in HMT buffer 1A in a 10-µl reaction. 5 µl of Anti-Mouse AlphaLISA acceptor beads (1:250) and 5 µl of Primary Antibody 7 (1:50) were added and incubation continued for 30 min. Streptavidin Donor beads (1:125) were added (10 µl) and mixture was further incubated for 15 min at RT before counts were measured.
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纯度
> 80 % SDS-PAGE.
Affinity purified. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
预测分子量
75 kDa including tags -
氨基酸
785 to 1210 -
标签
GST tag N-Terminus -
额外的序列信息
74.6 kDa including tag
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技术指标
Our Abpromise guarantee covers the use of ab80323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Ankyrin repeat containing protein
- Bat 8
- Bat8
see all -
功能
Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. -
组织特异性
Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow. -
序列相似性
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.
Contains 7 ANK repeats.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
结构域
The SET domain mediates interaction with WIZ.
The ANK repeats bind H3K9me1 and H3K9me2. -
翻译后修饰
Methylated at Lys-185; automethylated. -
细胞定位
Nucleus. Chromosome. Associates with euchromatic regions. Does not associate with heterochromatin. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab80323 尚未被引用在任何文献中。