Recombinant人EGF protein (ab91371)

概述

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab91371 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 生物活性Activity: The ED50 as determined by a cell proliferation assay using MTS assay kit with NHEK cells (normal human epidermal keratinocytes) was < 0.1 ng/ml, corresponding to a specific activity of < 1 x 107 units/mg.
  • 应用

    SDS-PAGE

    Western blot

    Functional Studies

  • 纯度> 95 % SDS-PAGE.
    ab91371 is highly purified by propriety chromatographic procedures and filter sterilized.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Preservative: None
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, 10mM Sodium phosphate, pH 7.2

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

常规信息

  • 别名
    • Beta urogastrone
    • EGF
    • EGF_HUMAN
    • Epidermal growth factor
    • HOMG4
    • OTTHUMP00000219721
    • OTTHUMP00000219722
    • Pro epidermal growth factor
    • URG
    • Urogastrone
    see all
  • 功能EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).
  • 组织特异性Expressed in kidney, salivary gland, cerebrum and prostate.
  • 疾病相关Hypomagnesemia 4
  • 序列相似性Contains 9 EGF-like domains.
    Contains 9 LDL-receptor class B repeats.
  • 翻译后修饰O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
  • 细胞定位Membrane.
  • Information by UniProt

Recombinant human EGF protein 图像

  • SDS-PAGE showing ab91371 at approximately 6.5kDa.

Recombinant human EGF protein (ab91371)参考文献

ab91371 has not yet been referenced specifically in any publications.

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The two active proteins are essentially the same, and both can be used to induce phosphorylation as they have been tested in functional studies. The only difference would be that ab9697 is supplied as lyophilized powde...

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