Recombinant人EB3 protein (ab101197)

概述

  • 产品名称Recombinant人EB3 protein
  • 蛋白长度Full length protein

描述

  • 性质Recombinant
  • 来源Escherichia coli
  • 氨基酸序列
    • AccessionQ9UPY8
    • 种属Human
    • 序列MGSSHHHHHH SSGLVPRGSH MAVNVYSTSV TSENLSRHDM LAWVNDSLHL NYTKIEQLCS GAAYCQFMDM LFPGCVHLRK VKFQAKLEHE YIHNFKVLQA AFKKMGVDKI IPVEKLVKGK FQDNFEFIQW FKKFFDANYD GKDYNPLLAR QGQDVAPPPN PGDQIFNKSK KLIGTAVPQR TSPTGPKNMQ TSGRLSNVAP PCILRKNPPS ARNGGHETDA QILELNQQLV DLKLTVDGLE KERDFYFSKL RDIELICQEH ESENSPVISG IIGILYATEE GFAPPEDDEI EEHQQEDQDE Y
    • 分子量34 kDa including tags
    • 氨基酸1 to 281
    • 标签His tag N-Terminus

技术指标

Our Abpromise guarantee covers the use of ab101197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度> 90 % SDS-PAGE.
    ab101197 is purified using conventional chromatography techniques.
  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    Preservative: None
    Constituents: 10% Glycerol, 20mM Tris HCl, 2mM DTT, 100mM Sodium chloride, pH 8

常规信息

  • 别名
    • APC binding protein
    • EB 3
    • EB1 protein family member 3
    • EB3
    • EBF 3
    • EBF3
    • EBF3 S
    • End binding protein 3
    • End-binding protein 3
    • MAPRE 3
    • MAPRE3
    • MARE3_HUMAN
    • Member 3
    • Microtubule Associated Protein
    • Microtubule associated protein RP/EB family member 3
    • Microtubule-associated protein RP/EB family member 3
    • OTTHUMP00000200910
    • RP 3
    • RP/EB Family
    • RP3
    see all
  • 功能May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
  • 组织特异性Predominantly expressed in brain and muscle.
  • 序列相似性Belongs to the MAPRE family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 EB1 C-terminal domain.
  • 结构域Composed of two functionally independent domains. The N-terminal domain forms an hydrophobic cleft involved in microtubule binding and the C-terminal is involved in the formation of mutually exclusive complexes with APC and DCTN1.
  • 细胞定位Cytoplasm > cytoskeleton. Associated with the microtubule network.
  • Information by UniProt

Recombinant Human EB3 protein 图像

  • 15% SDS-PAGE showing ab101197 at approximately 34.1kDa (3µg).

Recombinant Human EB3 protein (ab101197)参考文献

ab101197 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Unfortunately we do not sell DNA constructs.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal a...

Read More



Thanks for your enquiry. ab101197 is our only EB3 protein product. This product is a recombinant, full length protein produced in E. coli. The SDS PAGE gel does show the protein in its monomeric form as the gel format is denaturing and reduci...

Read More

We haven’t checked whether our protein is monomeric or dimeric.

However, we will verify it using FPLC, and within 10days we should have the results.
As soon as I have the FPLC data, I will let you know.

Thank you for contacting us.

The answers to your questions are as follows:

1) Why was DTT added?
- DTT is added because it helps stabilize proteins with free cysteines.

2) Is the protein stable without it?
- We ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"