Recombinant人E2F1 protein (ab82207)

概述

描述

  • 性质Recombinant
  • 来源Baculovirus infected insect cells
  • 氨基酸序列
    • 种属Human

技术指标

Our Abpromise guarantee covers the use of ab82207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    Functional Studies

    EMSA

  • 形式Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    Preservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0

常规信息

  • 别名
    • Dmel\CG6376
    • Dmel_CG6376
    • drosE2F1
    • E(Sev-CycE)3A
    • E(var)3-93E
    • E2-promoter binding facto
    • E2F 1
    • E2F transcription factor 1
    • E2F-1
    • E2f-PA
    • E2f-PB
    • E2f-PC
    • E2F1
    • E2f1 E2F transcription factor 1
    • E2F1_HUMAN
    • Evar(3)164
    • KIAA4009
    • l(3)07172
    • l(3)j3B1
    • l(3)j3C2
    • l(3)rM729
    • mKIAA4009
    • OTTHUMP00000030661
    • PBR3
    • PRB binding protein E2F 1
    • PRB-binding protein E2F-1
    • RBAP 1
    • RBAP-1
    • RBAP1
    • RBBP-3
    • RBBP3
    • RBP 3
    • RBP3
    • Retinoblastoma-associated protein 1
    • Retinoblastoma-binding protein 3
    • Transcription factor E2F1
    see all
  • 功能Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.
  • 序列相似性Belongs to the E2F/DP family.
  • 翻译后修饰Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase.
    Acetylation stimulates DNA-binding. Enhanced under stress conditions such as DNA damage and inhibited by retinoblastoma protein pRB. Regulated by KAP1/TRIM28 which recruits HDAC1 to E2F1 resulting in deacetylation. Acetylated by P/CAF/KAT2B.
  • 细胞定位Nucleus.
  • Information by UniProt

Recombinant Human E2F1 protein (ab82207)参考文献

ab82207 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry.

I suggest this protein will need to be reduced and denatured before loading onto the gel. Therefore, the customer will need to add sample buffer (containing SDS and mercaptoethanol) and boil for 5 minutes, as with...

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We unfotunaltery haven't tested this product in WB. Customer might have try to try the product themselves./ Please note that ab82207 is a protein not an antibody.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"