Recombinant人DNA Polymerase lambda protein (ab113399)

概述

描述

  • 性质
    Recombinant
  • 来源
    Escherichia coli
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      MGSSHHHHHHSSGLVPRGSHMLMHHQKYLQRFLGGKREKKQKEACSIPGI GKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQ GFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQA FNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEG FLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPT EKDVFRLLGLPYREPAERDW
    • 分子量
      36 kDa
    • 氨基酸
      1 to 300
    • 标签
      His tag N-Terminus
    • 额外的序列信息
      The protein sequence refers to isoform 2 and contains MGSSHHHHHHSSGLVPRGSH sequence at N-Terminus for the His tag.

技术指标

Our Abpromise guarantee covers the use of ab113399 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Mass Spectrometry

    SDS-PAGE

  • 质谱法
    MALDI-TOF
  • 纯度
    > 90 % SDS-PAGE.
    Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA).
  • 形式
    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 30% Glycerol

常规信息

  • 别名
    • BETA N
    • BETAN
    • DNA directed DNA polymerase lambda
    • DNA polymerase beta 2
    • DNA polymerase beta-2
    • DNA polymerase kappa
    • DNA polymerase kappa DNA polymerase beta N
    • DNA polymerase lambda
    • DNA polymerase lamda2
    • DPOLL_HUMAN
    • EC 2.7.7.7,EC 4.2.99.
    • FLJ46002
    • OTTHUMP00000020321
    • OTTHUMP00000020323
    • OTTHUMP00000059179
    • Pol beta2
    • POL KAPPA
    • Pol Lambda
    • POLKAPPA
    • POLL
    • Polymerase DNA directed lambda
    see all
  • 功能
    Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
  • 组织特异性
    Expressed in a number of tissues. Abundant in testis.
  • 序列相似性
    Belongs to the DNA polymerase type-X family.
    Contains 1 BRCT domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus.
  • Information by UniProt

图片

  • 15% SDS-PAGE of DNA Polymerase lambda protein: (1) MW ladder, (2) protein loaded 3ug

文献

ab113399 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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