来源 Escherichia coli
序列 MGSSHHHHHHSSGLVPRGSHMLMHHQKYLQRFLGGKREKKQKEACSIPGI GKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQMWYQQ GFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQKAAQA FNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEG FLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPT EKDVFRLLGLPYREPAERDW
分子量 36 kDa
氨基酸 1 to 300
标签 His tag N-Terminus
额外的序列信息 The protein sequence refers to isoform 2 and contains MGSSHHHHHHSSGLVPRGSH sequence at N-Terminus for the His tag. 技术指标
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
纯度 > 90
% SDS-PAGE. Conventional chromatography techniques (anion exchange followed by gel filtration with 20mM Tris pH 7.5, 2mM EDTA).
Concentration information loading... 制备和贮存
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.02% DTT, 0.32% Tris HCl, 0.58% Sodium chloride, 30% Glycerol
DNA directed DNA polymerase lambda
功能 Repair polymerase. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.
组织特异性 Expressed in a number of tissues. Abundant in testis.
序列相似性 Belongs to the DNA polymerase type-X family. Contains 1 BRCT domain.
翻译后修饰 Phosphorylated upon DNA damage, probably by ATM or ATR.
Information by UniProt
Recombinant Human DNA Polymerase lambda protein 图像
Recombinant Human DNA Polymerase lambda protein (ab113399)参考文献
has not yet been referenced specifically in any publications.
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