Recombinant人DNA polymerase alpha protein (ab114839)

概述

  • 产品名称
    Recombinant人DNA polymerase alpha protein
  • 蛋白长度
    Protein fragment

描述

  • 性质
    Recombinant
  • 来源
    Wheat germ
  • 氨基酸序列
    • Accession
    • 种属
      Human
    • 序列
      QFSRTGPLCPACMKATLQPEYSDKSLYTQLCFYRYIFDAECALEKLTTDH EKDKLKKQFFTPKVLQDYRKLKNTAEQFLSRSGYSEVNLSKLFAGCAVKS
    • 分子量
      37 kDa including tags
    • 氨基酸
      1363 to 1462

技术指标

Our Abpromise guarantee covers the use of ab114839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    ELISA

    SDS-PAGE

    Western blot

  • 形式
    Liquid
  • 补充说明
    Protein concentration is above or equal to 0.05 mg/ml.
    This protein is best used within three months from the date of receipt.
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

常规信息

  • 别名
    • DKFZp686K1672
    • DNA polymerase alpha 1 catalytic subunit
    • DNA polymerase alpha catalytic subunit
    • DNA polymerase alpha catalytic subunit p180
    • DNA polymerase alpha p180 subunit
    • DPOLA_HUMAN
    • OTTHUMP00000023089
    • OTTHUMP00000023090
    • P180
    • p180 subunit
    • POLA
    • POLA1
    • Polymerase (DNA directed), alpha 1, catalytic subunit
    • Polymerase (DNA-directed), alpha (70kD)
    see all
  • 功能
    Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.
  • 序列相似性
    Belongs to the DNA polymerase type-B family.
  • 翻译后修饰
    A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.
  • 细胞定位
    Nucleus.
  • Information by UniProt

Recombinant Human DNA polymerase alpha protein 图像

  • ab114839 analysed by 12.5% SDS-PAGE and stained with Coomassie Blue.

Recombinant Human DNA polymerase alpha protein (ab114839)参考文献

ab114839 has not yet been referenced specifically in any publications.

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